Microbial Community Analyses of the Deteriorated Storeroom Objects in the Tianjin Museum Using Culture-Independent and Culture-Dependent Approaches

Frontiers in Microbiology
Zijun LiuJiao Pan

Abstract

In the storeroom C7 of the Tianjin Museum, one wooden desk and two leather luggages dated back to Qing dynasty (1644-1912 AD) presented viable microbial contamination. The aim of the present study was to investigate microbial communities responsible for the biodeterioration of storeroom objects using a combination of culture-independent and culture-dependent methods as well microscopic techniques. Scanning electron microscopy (SEM) revealed that the microflora on three storeroom objects were characterized by a marked presence of Eurotium halophilicum. Real-time quantitative polymerase chain reaction (qPCR) analysis proved that fungi were the main causative agents behind the biodeterioration in this case. Fungal internal transcribed spacer (ITS) amplicon sequencing documented the presence of two main fungi - Eurotium halophilicum and Aspergillus penicillioides. Molecular identification of fungal strains isolated from the surfaces and the air of the storeroom were most closely related to Chaetomium, Aspergillus, Penicillium, and Fusarium, showing discrepancies in fungal taxa compared to ITS amplicon sequencing. The most isolated bacterial phylum was Firmicutes, mostly Bacillus members. In addition, four biocide products - Prevent...Continue Reading

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Citations

Oct 3, 2019·World Journal of Microbiology & Biotechnology·Yunchuan LongShaoqi Zhou

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Datasets Mentioned

BETA
MH171483
KX379227
KT876643
KM979775
GU985086

Methods Mentioned

BETA
electrophoresis
amplicon sequencing
scanning electron microscopy
PCR
Illumina sequencing

Software Mentioned

BLAST
Uparse
GENEWIZ
QIIME
Molecular Evolutionary Genetics Analysis ( MEGA
Clustal W
MEGA

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