Microbial Community Analysis of Sauerkraut Fermentation Reveals a Stable and Rapidly Established Community

Foods
Michelle A ZabatPeter Belenky

Abstract

Despite recent interest in microbial communities of fermented foods, there has been little inquiry into the bacterial community dynamics of sauerkraut, one of the world’s oldest and most prevalent fermented foods. In this study, we utilize 16S rRNA amplicon sequencing to profile the microbial community of naturally fermented sauerkraut throughout the fermentation process while also analyzing the bacterial communities of the starting ingredients and the production environment. Our results indicate that the sauerkraut microbiome is rapidly established after fermentation begins and that the community is stable through fermentation and packaging for commercial sale. Our high-throughput analysis is in agreement with previous studies that utilized traditional microbiological assessments but expands the identified taxonomy. Additionally, we find that the microbial communities of the starting ingredients and the production facility environment exhibit low relative abundance of the lactic acid bacteria that dominate fermented sauerkraut.

References

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Aug 9, 2005·International Journal of Food Microbiology·Myungjin Kim, Jongsik Chun
Oct 9, 2007·Applied and Environmental Microbiology·Vethachai PlengvidhyaHenry P Fleming
Nov 21, 2007·International Journal of Food Microbiology·Luca Settanni, Aldo Corsetti
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Nov 2, 2017·Nature·Luke R ThompsonUNKNOWN Earth Microbiome Project Consortium
May 1, 2018·Food Microbiology·Michelle A ZabatPeter Belenky

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Citations

Apr 21, 2019·Applied and Environmental Microbiology·Esther R MillerBenjamin E Wolfe
Jul 11, 2020·Critical Reviews in Food Science and Nutrition·Pierre LedormandMarion Dalmasso
Aug 14, 2019·Metabolites·Ruma RaghuvanshiRobert A Quinn
Apr 11, 2019·Frontiers in Microbiology·Fernando Astudillo-MelgarGerardo Huerta-Beristain
Jan 23, 2021·Critical Reviews in Food Science and Nutrition·Laila N ShwaikiKieran M Lynch
Nov 19, 2020·Foods·Theodoros Varzakas
Jan 6, 2021·Nature Reviews. Gastroenterology & Hepatology·Maria L MarcoRobert Hutkins
Jul 13, 2021·Current Opinion in Biotechnology·Jennifer Mahony, Douwe van Sinderen

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Datasets Mentioned

BETA
SRP145097

Methods Mentioned

BETA
amplicon sequencing
PCR

Software Mentioned

MAFFT
QIIME2
Quantitative Insights Into Microbial Ecology 2
Prism
FastTree

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