Microbial community and ovine host response varies with early and late stages of Haemonchus contortus infection

Veterinary Research Communications
Saeed El-AshramXun Suo

Abstract

The interactions between gastric microbiota, ovine host, and Haemonchus contortus portray the ovine gastric environment as a complex ecosystem, where all factors play a pertinent role in fine-tuning each other and in haemeostasis. We delineated the impact of early and late Haemonchus infection on abomasal and ruminal microbial community, as well as the ovine host. Twelve, parasite-naive lambs were divided into four groups, 7 days post-infection (dpi) and time-matched uninfected-control groups; 50 dpi and time-matched uninfected control groups were used for the experiment. Six sheep were inoculated with 5000 H. contortus infective larvae and followed for 7 or 50 days with their corresponding uninfected-control ones. Ovine abomasal tissues were collected for histological analysis and gastric fluids were collected for PH value measurements, microbial community isolation and Illumina MiSeq platform and bioinformatic analysis. Our results showed that Haemonchus infection increased the abomasal gastric pH (P = 0.05) and resulted in necrotizing and inflammatory changes that were more severe during acute infection. Furthermore, infection increased the abomasal bacterial load and decreased the ruminal microbiome. A 7-day infection of sh...Continue Reading

References

Mar 1, 1990·Veterinary Parasitology·W A Rahman, G H Collins
Oct 1, 1985·International Journal for Parasitology·K M Dash
Dec 1, 1982·Experimental Parasitology·C Honde, L Bueno
Aug 1, 1993·International Journal for Parasitology·P G Douch, P E Morum
Aug 1, 1996·International Journal for Parasitology·P G DouchR G Windon
Jul 1, 1997·International Journal for Parasitology·H V SimpsonW E Pomroy
Sep 29, 1999·International Journal for Parasitology·D C SimcockH V Simpson
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M D SäemannG J Zlabinger
Apr 1, 2004·Canadian Journal of Gastroenterology = Journal Canadien De Gastroenterologie·Jennifer L ShinGabor Kandel
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Apr 4, 2006·Journal of Animal Science·J E Miller, D W Horohov
Dec 8, 2010·Future Microbiology·Corentine AlauzetAlain Lozniewski
Apr 29, 2011·Parasitology Research·Jaroslav VadlejchMilan Moravec
Sep 3, 2011·Science·Gary D WuJames D Lewis
Oct 4, 2011·BMC Bioinformatics·Brian D OndovAdam M Phillippy
Mar 24, 2012·Animal : an International Journal of Animal Bioscience·A IdrisM Gauly
Nov 20, 2012·Frontiers in Cellular and Infection Microbiology·Federica BerrilliStefano D'Amelio
Apr 2, 2013·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·G BordoloiJ D Ghosh
Aug 9, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jordan L PlieskattPaul J Brindley
Nov 28, 2013·GigaScience·Yoshiki Vázquez-BaezaRob Knight
Jan 16, 2014·BMC Bioinformatics·Jianlong QiTom Michoel
May 6, 2014·The Journal of Infectious Diseases·Cinzia CantacessiAlex Loukas
May 24, 2014·PLoS Neglected Tropical Diseases·Soo Ching LeeP'ng Loke
Jul 19, 2014·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Javad JavanbakhtSamad Alimohammadi
Aug 7, 2014·Parasites & Vectors·Guilherme Costa FaustoMarta Lizandra do Rêgo Leal
Mar 3, 2015·Veterinary Parasitology·Diana GassóEmmanuel Serrano
Sep 9, 2015·Parasite Immunology·Mario M Zaiss, Nicola L Harris

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Citations

Apr 17, 2020·Animals : an Open Access Journal From MDPI·Asavela NgalavuThobela Louis Tyasi
Aug 21, 2019·BMC Veterinary Research·Dominika MravčákováMarián Várady
Oct 14, 2019·Parasitology Research·Saeed A El-AshramKhaled T Abdelaziz
Jun 13, 2020·Expert Review of Anti-infective Therapy·Alba CortésCinzia Cantacessi
Jun 27, 2019·The ISME Journal·Glenn HoganDave J Bartley
Mar 3, 2020·Current Opinion in Microbiology·Thomas J SharptonMichael Kent

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Datasets Mentioned

BETA
X78803.1

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

FLASH
Emperor
house Perl scripts
R
UniFrac
stat
Instat
Qiime
WGCNA
ggplot2

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