Microbial community dynamics and effect of environmental microbial reservoirs on red-backed salamanders (Plethodon cinereus)

The ISME Journal
Andrew H LoudonReid N Harris

Abstract

Beneficial cutaneous bacteria on amphibians can protect against the lethal disease chytridiomycosis, which has devastated many amphibian species and is caused by the fungus Batrachochytrium dendrobatidis. We describe the diversity of bacteria on red-backed salamanders (Plethodon cinereus) in the wild and the stability of these communities through time in captivity using culture-independent Illumina 16S rRNA gene sequencing. After field sampling, salamanders were housed with soil from the field or sterile media. The captive conditions led to different trajectories of bacterial communities. Eight OTUs present on >90% of salamanders in the field, through time, and in both treatments were defined as the core community, suggesting that some bacteria are closely associated with the host and are independent of an environmental reservoir. One of these taxa, a Pseudomonas sp., was previously cultured from amphibians and found to be antifungal. As all host-associated bacteria were found in the soil reservoir, environmental microbes strongly influence host-microbial diversity and likely regulate the core community. Using PICRUSt, an exploratory bioinformatics tool to predict gene functions, we found that core skin bacteria provided simila...Continue Reading

References

Jun 17, 1998·Proceedings of the National Academy of Sciences of the United States of America·W B WhitmanW J Wiebe
Jul 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·L BergerH Parkes
Oct 7, 2004·Journal of Wildlife Diseases·Seanna L AnnisJoyce E Longcore
Mar 11, 2005·Nature Reviews. Microbiology·Dieter Haas, Geneviève Défago
Feb 17, 2006·Proceedings of the National Academy of Sciences of the United States of America·Karen R LipsJames P Collins
Aug 23, 2006·Ecology·Lara J RachowiczCheryl J Briggs
Mar 27, 2007·Nature Reviews. Microbiology·Eugene RosenbergIlana Zilber-Rosenberg
Jul 3, 2007·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·M A ZoccoA Gasbarrini
Oct 19, 2007·Nature·Peter J TurnbaughJeffrey I Gordon
Mar 24, 2009·Diseases of Aquatic Organisms·Reid N HarrisRoss A Alford
Mar 27, 2009·The ISME Journal·Reid N HarrisKevin P C Minbiole
Apr 21, 2009·Molecular Biology and Evolution·Morgan N PriceAdam P Arkin
Jun 9, 2009·Applied and Environmental Microbiology·Christian L LauberNoah Fierer
Sep 1, 2009·Applied and Environmental Microbiology·Matthew H BeckerKevin P C Minbiole
Nov 17, 2009·Bioinformatics·J Gregory CaporasoRob Knight
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·J Gregory CaporasoRob Knight
Jul 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Andrew J CrawfordEldredge Bermingham
May 27, 2011·American Journal of Botany·Noah Fierer, Jay T Lennon
Jun 1, 2011·Genome Biology·J Gregory CaporasoRob Knight
Jun 4, 2011·Bioinformatics·Simon A Berger, Alexandros Stamatakis
Oct 1, 2011·The ISME Journal·Valerie J McKenzieChristian L Lauber
Jan 5, 2012·PloS One·Niels O VerhulstRenate C Smallegange
Jan 24, 2012·Soil Biology & Biochemistry·Gaddy T BergmannNoah Fierer
Dec 4, 2012·Nature Methods·Nicholas A BokulichJ Gregory Caporaso
Jan 9, 2013·International Journal of Antimicrobial Agents·Grégory DubourgDidier Raoult
Apr 1, 2011·Environmental Microbiology Reports·Amy ApprillMichael S Rappé
Aug 27, 2013·Nature Biotechnology·Morgan G I LangilleCurtis Huttenhower

❮ Previous
Next ❯

Citations

Jan 31, 2016·Journal of Chemical Ecology·Ananda Brito de AssisCristine Chaves Barreto
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Andrea J Jani, Cheryl J Briggs
Aug 13, 2015·Experimental & Applied Acarology·Ralph A SaporitoThomas F Spande
Mar 1, 2016·Die Naturwissenschaften·Miguel VencesJulian Glos
Mar 31, 2015·Frontiers in Immunology·Bruno M ColomboNicolas Pollet
Aug 16, 2016·The Science of the Total Environment·Sara CostaPaula V Morais
Sep 2, 2016·Frontiers in Microbiology·Huan LiXiangzhen Li
Oct 1, 2016·PeerJ·Virginie Lemieux-LabontéFrançois-Joseph Lapointe
Sep 23, 2016·Proceedings. Biological Sciences·Jordan G KuenemanValerie J McKenzie
Sep 30, 2016·Microbial Ecology·Obed Hernández-GómezRod N Williams
Sep 9, 2016·PLoS Pathogens·Jenifer B Walke, Lisa K Belden
Apr 19, 2017·Environmental Microbiology·Joana Sabino-PintoMiguel Vences
Jul 6, 2017·Environmental Microbiology·Jenifer B WalkeLisa K Belden
Jan 25, 2018·The ISME Journal·Catalina Cuellar-Gempeler, Mathew A Leibold
Oct 7, 2017·Integrative and Comparative Biology·Valerie J McKenzieRob Knight
Nov 19, 2015·MBio·Alexandra DostalChristophe Chassard
Feb 19, 2017·Applied and Environmental Microbiology·Carly R Muletz-WolzKaren R Lips

❮ Previous
Next ❯

Datasets Mentioned

BETA
ERP003771

Methods Mentioned

BETA
PCR
soil treatment

Software Mentioned

Greengenes
PiCRUSt
PRIMER
PyNAST
RAxML
UniFrac
FastTree
SPSS
QIIME
Principal Coordinates Analysis ( PCoA )

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.