Microbial Community Response to Simulated Petroleum Seepage in Caspian Sea Sediments

Frontiers in Microbiology
Marion StagarsKatrin Knittel

Abstract

Anaerobic microbial hydrocarbon degradation is a major biogeochemical process at marine seeps. Here we studied the response of the microbial community to petroleum seepage simulated for 190 days in a sediment core from the Caspian Sea using a sediment-oil-flow-through (SOFT) system. Untreated (without simulated petroleum seepage) and SOFT sediment microbial communities shared 43% bacterial genus-level 16S rRNA-based operational taxonomic units (OTU0.945) but shared only 23% archaeal OTU0.945. The community differed significantly between sediment layers. The detection of fourfold higher deltaproteobacterial cell numbers in SOFT than in untreated sediment at depths characterized by highest sulfate reduction rates and strongest decrease of gaseous and mid-chain alkane concentrations indicated a specific response of hydrocarbon-degrading Deltaproteobacteria. Based on an increase in specific CARD-FISH cell numbers, we suggest the following groups of sulfate-reducing bacteria to be likely responsible for the observed decrease in aliphatic and aromatic hydrocarbon concentration in SOFT sediments: clade SCA1 for propane and butane degradation, clade LCA2 for mid- to long-chain alkane degradation, clade Cyhx for cycloalkanes, pentane an...Continue Reading

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Datasets Mentioned

BETA
SRP070456
CAO03074
LT546441
LT546456

Methods Mentioned

BETA
PCR
PCRs
Chip
Fluorescence

Software Mentioned

SILVA
UCHIME
GEOMAR
MiSeq
BBmap
ANOSIM
R
BaseCaller
SILVAngs
mothur

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