Microbial Community Structure and Functional Potential in Cultivated and Native Tallgrass Prairie Soils of the Midwestern United States

Frontiers in Microbiology
Rachel MackelprangJanet K Jansson

Abstract

The North American prairie covered about 3.6 million-km2 of the continent prior to European contact. Only 1-2% of the original prairie remains, but the soils that developed under these prairies are some of the most productive and fertile in the world, containing over 35% of the soil carbon in the continental United States. Cultivation may alter microbial diversity and composition, influencing the metabolism of carbon, nitrogen, and other elements. Here, we explored the structure and functional potential of the soil microbiome in paired cultivated-corn (at the time of sampling) and never-cultivated native prairie soils across a three-states transect (Wisconsin, Iowa, and Kansas) using metagenomic and 16S rRNA gene sequencing and lipid analysis. At the Wisconsin site, we also sampled adjacent restored prairie and switchgrass plots. We found that agricultural practices drove differences in community composition and diversity across the transect. Microbial biomass in prairie samples was twice that of cultivated soils, but alpha diversity was higher with cultivation. Metagenome analyses revealed denitrification and starch degradation genes were abundant across all soils, as were core genes involved in response to osmotic stress, res...Continue Reading

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Citations

Jan 10, 2019·FEMS Microbiology Ecology·Petr Baldrian
Mar 14, 2020·FEMS Microbiology Ecology·Fei ZhengYong-Guan Zhu
Jun 13, 2019·MSystems·Taniya Roy ChowdhuryJanet K Jansson
Apr 14, 2021·Journal of Environmental Management·A SinghS Dixit
Aug 5, 2021·Scientific Data·Vojtěch TláskalPetr Baldrian
Sep 24, 2021·Environmental Microbiology·Gyuhyon ChaKonstantinos T Konstantinidis

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Methods Mentioned

BETA
PCR
Phylogenetic Profiles

Software Mentioned

R
UBLAST
hmmalign
HMMER3
hmmbuild
cnygc
BLASTP
PERMANOVA
FastTree
USEARCH

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