Microbial Degradation of Lobster Shells to Extract Chitin Derivatives for Plant Disease Management

Frontiers in Microbiology
Gayathri IlangumaranBalakrishnan Prithiviraj

Abstract

Biodegradation of lobster shells by chitinolytic microorganisms are an environment safe approach to utilize lobster processing wastes for chitin derivation. In this study, we report degradation activities of two microbes, "S223" and "S224" isolated from soil samples that had the highest rate of deproteinization, demineralization and chitinolysis among ten microorganisms screened. Isolates S223 and S224 had 27.3 and 103.8 protease units mg-1 protein and 12.3 and 11.2 μg ml-1 of calcium in their samples, respectively, after 1 week of incubation with raw lobster shells. Further, S223 contained 23.8 μg ml-1 of N-Acetylglucosamine on day 3, while S224 had 27.3 μg ml-1 on day 7 of incubation with chitin. Morphological observations and 16S rDNA sequencing suggested both the isolates were Streptomyces. The culture conditions were optimized for efficient degradation of lobster shells and chitinase (∼30 kDa) was purified from crude extract by affinity chromatography. The digested lobster shell extracts induced disease resistance in Arabidopsis by induction of defense related genes (PR1 > 500-fold, PDF1.2 > 40-fold) upon Pseudomonas syringae and Botrytis cinerea infection. The study suggests that soil microbes aid in sustainable bioconver...Continue Reading

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Citations

Mar 5, 2018·Applied Microbiology and Biotechnology·Jelena SpasicJasmina Nikodinovic-Runic
Oct 22, 2019·The Plant Pathology Journal·Sruti BajpaiBalakrishnan Prithiviraj
Oct 24, 2020·International Journal of Biological Macromolecules·Yang ZhouDaolong Dou
Jul 23, 2021·3 Biotech·Gincy Marina MathewAshok Pandey

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Datasets Mentioned

BETA
KY818664
KY818663

Methods Mentioned

BETA
electrophoresis
PCR
Atomic absorption spectroscopy

Software Mentioned

BLAST ( Basic Local Alignment Search Tool )
SAS

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