Microbial Degradation of Phenanthrene in Pristine and Contaminated Sandy Soils

Microbial Ecology
Alexandra SchwarzEsmaeil Shahsavari

Abstract

Phenanthrene mineralisation studies in both pristine and contaminated sandy soils were undertaken through detailed assessment of the activity and diversity of the microbial community. Stable isotope probing (SIP) was used to assess and identify active 13C-labelled phenanthrene degraders. Baseline profiling indicated that there was little difference in fungal diversity but a significant difference in bacterial diversity dependent on contamination history. Identification of dominant fungal and bacterial species highlighted the presence of organisms capable of degrading various petroleum-based compounds together with other anthropogenic compounds, regardless of contamination history. Community response following a simulated contamination event (14C-phenanthrene) showed that the microbial community in deep pristine and shallow contaminated soils adapted most to the presence of phenanthrene. The similarity in microbial community structure of well-adapted soils demonstrated that a highly adaptable fungal community in these soils enabled a rapid response to the introduction of a contaminant. Ten fungal and 15 bacterial species were identified as active degraders of phenanthrene. The fungal degraders were dominated by the phylum Basidi...Continue Reading

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Datasets Mentioned

BETA
HE863776.1
KF911342.1
KF468222.1
AB105353.1
EF060778.1
AF472627.1
HF558623.1
KC445196.1
EU841705.1
GQ471877.1

Methods Mentioned

BETA
electrophoresis
density-gradient centrifugation
PCR

Software Mentioned

SIP
UPGMA
TotalLab

Related Concepts

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