Microbial dysbiosis and polyamine metabolism as predictive markers for early detection of pancreatic cancer.

Carcinogenesis
Roberto MendezSantanu Banerjee

Abstract

The lack of tools for early detection of pancreatic ductal adenocarcinoma (PDAC) is directly correlated with the abysmal survival rates in patients. In addition to several potential detection tools under active investigation, we tested the gut microbiome and its metabolic complement as one of the earliest detection tools that could be useful in patients at high risk for PDAC. We used a combination of 16s rRNA pyrosequencing and whole-genome sequencing of gut fecal microbiota in a genetically engineered PDAC murine model (KRASG12DTP53R172HPdxCre or KPC). Metabolic reconstruction of microbiome was done using the HUMAnN2 pipeline. Serum polyamine levels were measured from murine and patient samples using chromogenic assay. Our results showed a Proteobacterial and Firmicutes dominance in gut microbiota in early stages of PDAC development. Upon in silico reconstruction of active metabolic pathways within the altered microbial flora, polyamine and nucleotide biosynthetic pathways were significantly elevated. These metabolic products are known to be actively assimilated by the host and eventually utilized by rapidly dividing cells for proliferation validating their importance in the context of tumorigenesis. In KPC mice, as well as PD...Continue Reading

References

Nov 13, 2007·Gastroenterology Clinics of North America·Ralph H HrubanMichael Goggins
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Jun 16, 2012·Nature·UNKNOWN Human Microbiome Project Consortium
Jun 22, 2012·PLoS Computational Biology·Sahar AbubuckerCurtis Huttenhower
Jan 10, 2013·PLoS Computational Biology·Xochitl C Morgan, Curtis Huttenhower
Jan 17, 2013·Genome Biology·Todd J TreangenMihai Pop
Jul 23, 2013·International Journal of Medical Microbiology : IJMM·Maria Letizia Di MartinoGianni Prosseda
Oct 12, 2013·Cancer Treatment and Research·Meredith A J HullarJohanna W Lampe
Jan 7, 2015·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Daniel P Faith
May 2, 2015·Pancreas·Suresh T ChariDavid Wong
Dec 19, 2015·European Journal of Dermatology : EJD·Alexander SalavaAntti Lauerma
Jan 1, 2016·Biochemical and Biophysical Research Communications·Ravi RanjanDavid L Perkins
May 6, 2016·Frontiers in Microbiology·Juan JovelGane K-S Wong
Jul 3, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Jun Miyoshi, Eugene B Chang
Nov 3, 2016·Cancer Research·Caroline H JohnsonGary Siuzdak
Dec 22, 2016·Scientific Reports·Emma BarnardHuiying Li
Mar 9, 2017·Nature Reviews. Gastroenterology & Hepatology·James L AlexanderJames M Kinross
Mar 14, 2017·Nutrition and Cancer·Heyao MaYoshihiro Hayakawa
Mar 25, 2017·Cancer Discovery
Mar 30, 2017·World Journal of Gastroenterology : WJG·Natalie Ertz-ArchambaultDaniel Von Hoff
May 22, 2017·Cancer Letters·Kosuke MimaHideo Baba
May 27, 2017·Experimental & Molecular Medicine·Donghyun KimGabriel Núñez
Aug 2, 2017·Cancer Causes & Control : CCC·Sara H OlsonRobert C Kurtz
Sep 19, 2017·Translational Research : the Journal of Laboratory and Clinical Medicine·Kimberly A KrautkramerHannah V Carey
Sep 22, 2017·PLoS Pathogens·Laura E FulbrightJanelle C Arthur
Nov 11, 2017·Microbiome·Shan LiSergey R Konstantinov
Nov 21, 2017·Clujul medical : revistă de medicină şi farmacie·Cristian Vasile PetraDan Lucian Dumitraşcu
Dec 5, 2017·Cancer Letters·Qixing MaoJianzhong Hu
Jan 30, 2018·Science Advances·Amaia Arruabarrena-AristorenaArkaitz Carracedo
Feb 7, 2018·Molecular & Cellular Oncology·Leore T Geller, Ravid Straussman
Feb 27, 2018·Gastroenterology Report·Shaomin ZouMong-Hong Lee
Mar 2, 2018·Human Vaccines & Immunotherapeutics·Audrey Humphries, Adil Daud
Apr 4, 2018·Cancer Discovery·Erick RiquelmeFlorencia McAllister

❮ Previous
Next ❯

Citations

Oct 12, 2019·Marine Drugs·Helen J FittonSamuel N Karpiniec
Sep 13, 2019·Journal of Clinical Medicine·Hui-Jen Tsai, Jeffrey S Chang
Aug 19, 2020·International Journal of Molecular Sciences·Tania RossiFrancesca Pirini
May 28, 2020·Cancers·Karolina Kaźmierczak-SiedleckaWojciech Makarewicz
Apr 30, 2020·Cancers·Borbála KissPéter Árkosy
Nov 6, 2020·Cancer Research·Sheetal Parida, Dipali Sharma
Feb 2, 2021·Frontiers in Oncology·Wenqing ZhangXiaoyu Li
Oct 24, 2020·Acta Pharmacologica Sinica·Li-Long PanJia Sun
Mar 7, 2021·Journal of Personalized Medicine·Beste TuranliRaghu Sinha
Apr 29, 2021·Gut·Vidhi Chandra, Florencia McAllister
May 30, 2021·Seminars in Cancer Biology·Gouri PandyaManoj Garg
Aug 8, 2021·Cancers·Mark StasiewiczTomasz M Karpiński
Aug 14, 2021·Current Opinion in Gastroenterology·Guru TrikudanathanShounak Majumder
Aug 30, 2021·Cancer Metastasis Reviews·Jenny Jing LiWen Wee Ma
Jul 2, 2021·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Robert HesterFlorencia McAllister
Nov 6, 2021·International Journal of Oncology·Xing HuangBole Tian

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.