Microbial ecology of the hive and pollination landscape: bacterial associates from floral nectar, the alimentary tract and stored food of honey bees (Apis mellifera)

PloS One
Kirk E AndersonVanessa Corby-Harris

Abstract

Nearly all eukaryotes are host to beneficial or benign bacteria in their gut lumen, either vertically inherited, or acquired from the environment. While bacteria core to the honey bee gut are becoming evident, the influence of the hive and pollination environment on honey bee microbial health is largely unexplored. Here we compare bacteria from floral nectar in the immediate pollination environment, different segments of the honey bee (Apis mellifera) alimentary tract, and food stored in the hive (honey and packed pollen or "beebread"). We used cultivation and sequencing to explore bacterial communities in all sample types, coupled with culture-independent analysis of beebread. We compare our results from the alimentary tract with both culture-dependent and culture-independent analyses from previous studies. Culturing the foregut (crop), midgut and hindgut with standard media produced many identical or highly similar 16S rDNA sequences found with 16S rDNA clone libraries and next generation sequencing of 16S rDNA amplicons. Despite extensive culturing with identical media, our results do not support the core crop bacterial community hypothesized by recent studies. We cultured a wide variety of bacterial strains from 6 of 7 phyl...Continue Reading

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Citations

Apr 18, 2014·PloS One·Vanessa Corby-HarrisKirk E Anderson
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Datasets Mentioned

BETA
EF187239

Methods Mentioned

BETA
PCR

Software Mentioned

EcoSim
Greengenes
Clearcut
Bellerophon
CLUSTAL
Mothur
BLASTn
RDP
Principle Coordinates Analysis ( PCoA )
MEGA

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