Microbial effects of part-stream low-frequency ultrasonic pretreatment on sludge anaerobic digestion as revealed by high-throughput sequencing-based metagenomics and metatranscriptomics

Biotechnology for Biofuels
Yu XiaTong Zhang

Abstract

Part-stream low-frequency ultrasound (LFUS) was one of the common practices for sludge disintegration in full-scale anaerobic digestion (AD) facilities. However, the effectiveness of part-stream LFUS treatment and its effect on AD microbiome have not been fully elucidated. Here we testified the effectiveness of part-stream LFUS pretreatment by treating only a fraction of feed sludge (23% and 33% total solid of the feed sludge) with 20 Hz LFUS for 70 s. State-of-the-art metagenomic and metatranscriptomic analysis was used to investigate the microbial process underpinning the enhanced AD performance by part-stream LFUS pretreatment. By pretreating 33% total solid of the feed sludge, methane yield was increased by 36.5%, while the volatile solid reduction ratio remained unchanged. RNA-seq of the microbiome at stable stage showed that the continuous dosage of easy-degradable LFUS-pretreated feed sludge had gradually altered the microbial community by selectingBacteroidaleshydrolyzer with greater metabolic capability to hydrolyze cellulosic biomass without substrate attachment. Meanwhile,Thermotogaleswith excellent cell mobility for nutrient capturing was highly active within the community. Foremost proportion of the methanogenesis ...Continue Reading

References

Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Sep 18, 2003·International Journal of Systematic and Evolutionary Microbiology·Susumu Asakawa, Kazunari Nagaoka
May 11, 2004·Microbiology·Alice C L LenNicholas A Jacques
Apr 7, 2005·Applied and Environmental Microbiology·Rakia ChouariAbdelghani Sghir
Jun 26, 2007·Applied and Environmental Microbiology·Qiong WangJames R Cole
Oct 4, 2007·International Journal of Systematic and Evolutionary Microbiology·Jingang CaiYan Feng
Jun 3, 2008·Nature Methods·Ali MortazaviBarbara Wold
Sep 30, 2008·Journal of Environmental Science and Health. Part A, Toxic/hazardous Substances & Environmental Engineering·Uwe NeisAnna Lundén
Apr 7, 2009·Bioinformatics·Ying HuangWeizhong Li
Sep 18, 2009·Water Science and Technology : a Journal of the International Association on Water Pollution Research·S Pérez-ElviraF Fdz-Polanco
Mar 31, 2010·Water Science and Technology : a Journal of the International Association on Water Pollution Research·S I Pérez-ElviraF Fdz-Polanco
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
Apr 21, 2010·Nucleic Acids Research·Wenhan ZhuMark Borodovsky
Jul 14, 2010·Applied and Environmental Microbiology·Carsten MettelWerner Liesack
Jul 27, 2010·Bioresource Technology·Onur Güven Apul, F Dilek Sanin
Sep 21, 2010·Nature Methods·Shaomei HePhilip Hugenholtz
Jan 22, 2011·Water Science and Technology : a Journal of the International Association on Water Pollution Research·É L Bordeleau, R L Droste
Jan 1, 2009·Standards in Genomic Sciences·Iain J AndersonNikos C Kyrpides
May 3, 2011·Waste Management·Azeem KhalidLorna Dawson
May 18, 2011·BMC Bioinformatics·Yuzhen YeHaixu Tang
Jun 22, 2011·Genome Research·Daniel H HusonStephan C Schuster
Nov 1, 2011·PLoS Computational Biology·Sean R Eddy
Jan 11, 2012·Bioscience, Biotechnology, and Biochemistry·Elena V KulaginaAndrei N Shkoporov
Jul 19, 2012·Annual Review of Microbiology·Jessica R SieberRobert P Gunsalus
Dec 1, 2012·Nucleic Acids Research·Daniel H HaftErin Beck
Aug 1, 2011·Environmental Microbiology Reports·Maria WesterholmAnna Schnürer
Nov 30, 2013·Nucleic Acids Research·Robert D FinnMarco Punta
Oct 21, 2014·Biotechnology for Biofuels·Linn SolliAnne Gunn Rike
Oct 31, 2014·Bioinformatics·Nicolas TerraponBernard Henrissat

❮ Previous
Next ❯

Methods Mentioned

BETA
Illumina sequencing
RNA-seq

Software Mentioned

HMMER
PhyloPythiaS
rapsearch
rRNA
QIIME
PhyML3
MetaGeneMark
MG
MEGAN5
RSEM

Related Concepts

Related Feeds

Alzheimer's Disease: Transcription

Impaired transcription is associated with the pathogenesis and progression of conditions such as Alzheimer's disease (AD). Here are the latest discoveries pertaining to transcription and AD.

Alzheimer's Disease: RNA Sequencing

RNA sequencing studies have shed light on the genetic and molecular mechanisms related to the pathophysiology of Alzheimer's disease (AD). This feed follows papers using RNA sequencing technologies in Alzheimer's research.

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.