Microbial enrichment and meta-omics analysis identify CAZymes from mangrove sediments with unique properties.

Enzyme and Microbial Technology
Douglas Antonio Alvaredo PaixãoFabio Marcio Squina

Abstract

Although lignocellulose is the most abundant and renewable natural resource for biofuel production, its use remains under exploration because of its highly recalcitrant structure. Its deconstruction into sugar monomers is mainly driven by carbohydrate-active enzymes (CAZymes). To develop highly efficient and fast strategies to discover biomass-degrading enzymes for biorefinery applications, an enrichment process combined with integrative omics approaches was used to identify new CAZymes. The lignocellulolytic-enriched mangrove microbial community (LignoManG) established on sugarcane bagasse (SB) was enriched with lignocellulolytic bacteria and fungi such as Proteobacteria, Bacteroidetes, Basidiomycota, and Ascomycota. These microbial communities were able to degrade up to 55 % of the total SB, indicating the production of lignocellulolytic enzymes. Metagenomic analysis revealed that the LignoManG harbors 18.042 CAZyme sequences such as of cellulases, hemicellulases, carbohydrate esterases, and lytic polysaccharide monooxygenase. Similarly, our metaproteomic analysis depicted several enzymes from distinct families of different CAZy families. Based on the LignoManG data, a xylanase (coldXynZ) was selected, amplified, cloned, expr...Continue Reading

References

Feb 17, 2000·Trends in Biotechnology·C GerdayG Feller
Aug 29, 2002·Acta Crystallographica. Section D, Biological Crystallography·Filip Van PetegemJozef Van Beeumen
Jan 18, 2005·FEMS Microbiology Reviews·Tony CollinsGeorges Feller
Jun 8, 2006·Annual Review of Biochemistry·Khawar Sohail Siddiqui, Ricardo Cavicchioli
Apr 5, 2007·Nature Protocols·Andrej ShevchenkoMatthias Mann
Jan 25, 2008·BMC Bioinformatics·Yang Zhang
Jun 30, 2009·Protein Expression and Purification·Fabio M SquinaRolf A Prade
Mar 10, 2010·BMC Bioinformatics·Doug HyattLoren J Hauser
Apr 23, 2010·The New Phytologist·Kessy AbarenkovUrmas Kõljalg
May 18, 2010·Plant Physiology·Ruben VanholmeWout Boerjan
Dec 15, 2010·Annual Review of Marine Science·I C FellerM C Ball
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
May 31, 2012·Nucleic Acids Research·Yanbin YinYing Xu
Sep 18, 2013·Algorithms for Molecular Biology : AMB·Rayan Chikhi, Guillaume Rizk
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Nov 19, 2013·Systematic and Applied Microbiology·Hannah L WooKristen M DeAngelis
Jan 28, 2014·Extremophiles : Life Under Extreme Conditions·Xiaoshuang LiuZiduo Liu
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Jun 24, 2014·Biotechnology for Biofuels·Diego Javier JiménezJan Dirk van Elsas
Aug 1, 2014·Applied Microbiology and Biotechnology·Rosana GoldbeckFabio M Squina
Dec 31, 2014·Nature Methods·Jianyi YangYang Zhang
Jul 4, 2015·Journal of Food Science and Technology·Lyned D Lasrado, Muralikrishna Gudipati
Feb 26, 2016·Scientific Reports·Shuo JiaoGehong Wei
May 24, 2016·Nature Methods·Benjamin J CallahanSusan P Holmes
Jul 16, 2016·Applied Microbiology and Biotechnology·Diego Javier JiménezJan Dirk van Elsas
Sep 14, 2016·The Journal of Biological Chemistry·Bruna Medeia CamposFabio Marcio Squina
Oct 6, 2016·International Journal of Molecular Sciences·Ping ShengJiannan Ding
Jan 6, 2017·Biotechnology for Biofuels·Robson TramontinaFabio Marcio Squina
Apr 14, 2017·Microbiology and Molecular Biology Reviews : MMBR·Salvador LladóPetr Baldrian

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