Microbial function and genital inflammation in young South African women at high risk of HIV infection.

Microbiome
Arghavan AlisoltaniLindi Masson

Abstract

Female genital tract (FGT) inflammation is an important risk factor for HIV acquisition. The FGT microbiome is closely associated with inflammatory profile; however, the relative importance of microbial activities has not been established. Since proteins are key elements representing actual microbial functions, this study utilized metaproteomics to evaluate the relationship between FGT microbial function and inflammation in 113 young and adolescent South African women at high risk of HIV infection. Women were grouped as having low, medium, or high FGT inflammation by K-means clustering according to pro-inflammatory cytokine concentrations. A total of 3186 microbial and human proteins were identified in lateral vaginal wall swabs using liquid chromatography-tandem mass spectrometry, while 94 microbial taxa were included in the taxonomic analysis. Both metaproteomics and 16S rRNA gene sequencing analyses showed increased non-optimal bacteria and decreased lactobacilli in women with FGT inflammatory profiles. However, differences in the predicted relative abundance of most bacteria were observed between 16S rRNA gene sequencing and metaproteomics analyses. Bacterial protein functional annotations (gene ontology) predicted inflamma...Continue Reading

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Citations

Feb 10, 2021·Infection and Immunity·Smritee DabeeHeather B Jaspan

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Methods Mentioned

BETA
GTPase
phosphotransferase
ubiquitination
PCR
ELISA
acetylation
PCA
Assay

Key Resources (RRID) Mentioned

CVCL_6471

Software Mentioned

R package randomForest
Piphillin
R
ggplot2 R
DB
limma R package
SILVA
randomForest R
limma R
EnhancedVolcano

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