PMID: 3767568Jul 1, 1986Paper

Microbial metabolism of chlorosalicylates: effect of prolonged subcultivation on constructed strains

Archives of Microbiology
M A RubioH J Knackmuss

Abstract

The hybrid strain Pseudomonas sp. WR4016 was subcultivated with increasing concentrations of 5-chlorosalicylate (5----10 mM) as sole carbon source over a period of 9 months. At intervals of approximately 3 months derivative strains WR4017, WR4018 and WR4019 were isolated which exhibited higher growth rates and increased substrate tolerance. Comparative analysis of the turnover rates of the key enzymes in chlorosalicylate degradation showed that the adaptation process did not result from structural modifications of these proteins. Instead, balanced overproduction of the salicylate hydroxylase and catechol 1,2-dioxygenase prevented the accumulation of toxic chlorocatechols and accounted for the reduction of the doubling times with 4- or 5-chlorosalicylate. A comparative analysis of a genetically engineered chlorosalicylate degrader PL300-1 showed similar regulatory patterns as the most advanced isolate WR4019 from the adaptation series.

References

Mar 1, 1979·Applied and Environmental Microbiology·J HartmannH J Knackmuss
Sep 1, 1979·Applied and Environmental Microbiology·R L CrawfordT D Frick
Feb 1, 1979·Nature·W Reineke, H J Knackmuss
Jan 1, 1974·Archives of Microbiology·E DornH J Knackmuss
May 1, 1971·The Biochemical Journal·W C EvansH N Fernley
May 1, 1971·The Biochemical Journal·W C EvansJ I Davies
Feb 1, 1984·Applied and Environmental Microbiology·W Reineke, H J Knackmuss
May 1, 1981·Journal of Bacteriology·D K ChatterjeeA M Chakrabarty
Jan 1, 1980·Applied and Environmental Microbiology·A SchreiberH J Knackmuss
Jan 1, 1980·Applied and Environmental Microbiology·K H EngesserH J Knackmuss
Jul 1, 1982·Applied and Environmental Microbiology·U Schwien, E Schmidt

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