Microbial Metabolism Shifts Towards an Adverse Profile with Supplementary Iron in the TIM-2 In vitro Model of the Human Colon

Frontiers in Microbiology
Guus A M KortmanHarold Tjalsma

Abstract

Oral iron administration in African children can increase the risk for infections. However, it remains unclear to what extent supplementary iron affects the intestinal microbiome. We here explored the impact of iron preparations on microbial growth and metabolism in the well-controlled TNO's in vitro model of the large intestine (TIM-2). The model was inoculated with a human microbiota, without supplementary iron, or with 50 or 250 μmol/L ferrous sulfate, 50 or 250 μmol/L ferric citrate, or 50 μmol/L hemin. High resolution responses of the microbiota were examined by 16S rDNA pyrosequencing, microarray analysis, and metagenomic sequencing. The metabolome was assessed by fatty acid quantification, gas chromatography-mass spectrometry (GC-MS), and (1)H-NMR spectroscopy. Cultured intestinal epithelial Caco-2 cells were used to assess fecal water toxicity. Microbiome analysis showed, among others, that supplementary iron induced decreased levels of Bifidobacteriaceae and Lactobacillaceae, while it caused higher levels of Roseburia and Prevotella. Metagenomic analyses showed an enrichment of microbial motility-chemotaxis systems, while the metabolome markedly changed from a saccharolytic to a proteolytic profile in response to iron....Continue Reading

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Citations

Nov 30, 2016·Journal of Medicinal Food·Emanuel VamanuIonela Sarbu
Dec 16, 2016·Nutrition Reviews·Andrew M PrenticeJörg Spieldenner
Oct 6, 2018·The Journal of Pharmacology and Experimental Therapeutics·Wei Ling LauKatrine Whiteson
Oct 11, 2017·Journal of the Science of Food and Agriculture·Katarzyna Skrypnik, Joanna Suliburska
Sep 24, 2020·Nature Reviews. Nephrology·Denise MafraPeter Stenvinkel
Jul 9, 2020·Immunology·Ana Sousa GerósJoe N Frost
Feb 6, 2021·Gut Microbes·Yohannes SeyoumChristèle Humblot
May 13, 2021·Nutrition in Clinical Practice : Official Publication of the American Society for Parenteral and Enteral Nutrition·Marcia RibeiroDenise Mafra
Nov 7, 2019·Journal of Agricultural and Food Chemistry·Jenni FirrmanBradley Evans

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Datasets Mentioned

BETA
PRJEB6542

Methods Mentioned

BETA
NMR

Software Mentioned

GraphPad Prism
GraphPad
Topshim shim

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