Microbial pattern recognition causes distinct functional micro-RNA signatures in primary human monocytes.

PloS One
Robert HäslerPhilip Rosenstiel

Abstract

Micro-RNAs (miRNAs) are short, non-coding RNAs that regulate gene expression post transcriptionally. Several studies have demonstrated the relevance of miRNAs for a wide range of cellular mechanisms, however, the current knowledge on how miRNAs respond to relevant external stimuli, e.g. in disease scenarios is very limited. To generate a descriptive picture of the miRNA network associated to inflammatory responses, we quantified the levels of 330 miRNAs upon stimulation with a panel of pro-inflammatory components such as microbial pattern molecules (flagellin, diacylated lipopeptide lipopolysaccharide, muramyl dipeptide), infection with Listeria monocytogenes and TNF-α as pro-inflammatory control in primary human monocytes using real time PCR. As a result, we found distinct miRNA response clusters for each stimulus used. Additionally, we identified potential target genes of three selected miRNAs miR-129-5p, miR-146a and miR-378 which were part of PAMP-specific response clusters by transfecting THP1 monocytes with the corresponding pre- or anti-miRNAs and microfluidic PCR arrays. The miRNAs induced distinct transcriptomal signatures, e.g. overexpression of miRNA129-5p, which was selectively upregulated by the NOD2-elicitor MDP, ...Continue Reading

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Citations

May 4, 2013·Trends in Immunology·Daniel B Graham, Ramnik J Xavier
Apr 14, 2016·International Journal of Molecular Sciences·Yongwei QinQian Gao
Sep 15, 2014·Journal of Renal Nutrition : the Official Journal of the Council on Renal Nutrition of the National Kidney Foundation·Matthew B RivaraJonathan Himmelfarb
Dec 4, 2013·Inflammatory Bowel Diseases·Alice Y ChuangJohn H Kwon
Jun 1, 2013·PloS One·Volker GasslingRobert Häsler
Jun 6, 2014·Oncotarget·Lixia WangZhiwei Wang

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Methods Mentioned

BETA
PCR
transfection
transfections
transfecting

Software Mentioned

TargetScanS
MedianIQR
Spotfire DSMA
PicTar
miRanda
Target Scan
PITA
UPGMA

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