Abstract
An analytical procedure is presented for obtaining detailed characterization of petroleum hydrocarbons which undergo microbial degradation. The procedure includes column chromatographic separation and characterization of the resulting fractions by mass spectrometry and gas chromatography. The use of computerized low-resolution mass spectrometry is offered as a method for assessing microbial degradation of petroleum. This method provides information which cannot, at the present time, be obtained by other available analytical methods. Use of this method to evaluate degradation of a South Louisiana crude oil by a mixed culture of estuarine bacteria revealed that asphaltenes and resins increased by 28% after degradation, while saturates and aromatics decreased by 83.4% and 70.5%, respectively. Most of the normal and branched-chain alkanes were degraded (96.4%), but an increase in long-chain alkanes (C28-C32) after degradation was observed by gas-liquid chromatography. Susceptibility of cycloalkanes to degradation was less as the structure varied, i.e., 6-ring greater than 1-ring greater than 2-ring greater than 3-ring greater than 5-ring greater than 4-ring. Susceptibility of aromatic components to degradation decreased with increa...Continue Reading
Citations
Aug 1, 1996·Biodegradation·L E RuddL D Claxton
Mar 20, 2002·Folia Microbiologica·C O ObuekweS S Radwan
Oct 1, 1996·Analytical and Bioanalytical Chemistry·V RiisM Möder
May 24, 2011·Biodegradation·G D Gojgic-CvijovicM M Vrvic
Apr 3, 2001·Applied and Environmental Microbiology·S MishraB Lal
Jan 1, 2011·Journal of Environmental Sciences (China)·Xinxin WangGuoqiang Zhuang
Sep 29, 1978·Annals of the New York Academy of Sciences·M J VollR R Colwell
Sep 1, 1977·CRC Critical Reviews in Microbiology·R R Colwell
Oct 1, 1996·The Journal of Applied Bacteriology·B Lal, S Khanna
Jul 31, 2004·Canadian Journal of Microbiology·Priyangshu Manab SarmaBanwari Lal
Jul 25, 1998·Journal of Chromatography. a·Z WangD W Westlake
Mar 1, 1981·Microbiological Reviews·R M Atlas
Apr 1, 1984·Applied and Environmental Microbiology·P M Fedorak, D W Westlake
Jun 1, 1989·Applied and Environmental Microbiology·J P Pendrys
Mar 1, 1981·Applied and Environmental Microbiology·R C Wyndham, J W Costerton
Dec 1, 1975·Applied Microbiology·J D WalkerL Petrakis
Sep 1, 1990·Microbiological Reviews·J G Leahy, R R Colwell
Feb 25, 2014·Environmental Science & Technology·Karin L LemkauChristopher M Reddy