Microbial prevalence, diversity and abundance in amniotic fluid during preterm labor: a molecular and culture-based investigation.

PloS One
Daniel B DiGiulioDavid A Relman

Abstract

Preterm delivery causes substantial neonatal mortality and morbidity. Unrecognized intra-amniotic infections caused by cultivation-resistant microbes may play a role. Molecular methods can detect, characterize and quantify microbes independently of traditional culture techniques. However, molecular studies that define the diversity and abundance of microbes invading the amniotic cavity, and evaluate their clinical significance within a causal framework, are lacking. In parallel with culture, we used broad-range end-point and real-time PCR assays to amplify, identify and quantify ribosomal DNA (rDNA) of bacteria, fungi and archaea from amniotic fluid of 166 women in preterm labor with intact membranes. We sequenced up to 24 rRNA clones per positive specimen and assigned taxonomic designations to approximately the species level. Microbial prevalence, diversity and abundance were correlated with host inflammation and with gestational and neonatal outcomes. Study subjects who delivered at term served as controls. The combined use of molecular and culture methods revealed a greater prevalence (15% of subjects) and diversity (18 taxa) of microbes in amniotic fluid than did culture alone (9.6% of subjects; 11 taxa). The taxa detected ...Continue Reading

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Dec 6, 1990·The New England Journal of Medicine·D A RelmanL S Tompkins
Oct 13, 1988·The New England Journal of Medicine·S L HillierD A Eschenbach
Jan 1, 1974·Archives of Neurology·R T Johnson, C J Gibbs
Jan 1, 1982·The Journal of Infectious Diseases·R S GibbsY S Castaneda
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P A HanffL H Moore
Aug 1, 1993·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A BlanchardG H Cassell
Jan 1, 1996·Clinical Microbiology Reviews·D N Fredricks, D A Relman
Jul 1, 1996·British Journal of Obstetrics and Gynaecology·J JalavaA Alanen
Jun 1, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·J HittiD A Eschenbach
Jan 10, 1998·American Journal of Obstetrics and Gynecology·G R MarkensonM Yancey
Feb 6, 1998·FEMS Microbiology Reviews·F von WintzingerodeE Stackebrandt
May 30, 1998·Archives of Pediatrics & Adolescent Medicine·J M LorenzN Paneth
Jul 31, 1998·The New England Journal of Medicine·R L Goldenberg, D J Rouse
Aug 26, 1998·Emerging Infectious Diseases·D A Relman
Dec 29, 1998·The New England Journal of Medicine·J A McGregor, J I French
May 18, 2000·The New England Journal of Medicine·R L GoldenbergW W Andrews
Aug 25, 2001·Paediatric and Perinatal Epidemiology·R RomeroY M Kim
Sep 26, 2001·Applied and Environmental Microbiology·J B HughesB J Bohannan
Feb 28, 2002·Genome Biology·Philip Hugenholtz
Mar 20, 2003·Swiss Medical Weekly·H U BucherUNKNOWN Swiss Neonatal Network
Jul 16, 2003·American Journal of Obstetrics and Gynecology·Harald LeitichPeter Husslein
Nov 11, 2003·Journal of Microbiological Methods·G C BakerD A Cowan
Jan 8, 2004·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Raymond W RedlineUNKNOWN Society for Pediatric Pathology, Perinatal Section, Amniotic Fluid Infection Nosology Committee
Aug 18, 2004·American Journal of Perinatology·Carolyn GardellaDavid Eschenbach
Nov 20, 2004·The Journal of Infectious Diseases·Jennifer K HebbPatricia A Totten
Feb 22, 2005·Oral Microbiology and Immunology·J KatsoulisG R Persson
Mar 9, 2005·Lancet·Joy E LawnUNKNOWN Lancet Neonatal Survival Steering Team
Apr 16, 2005·Science·Paul B EckburgDavid A Relman
Jul 12, 2005·Molecular and Cellular Probes·Jongyoun YiEui-Chong Kim
Jul 14, 2005·Emerging Infectious Diseases·Vijay A K B GundiBernard La Scola
Sep 10, 2005·American Journal of Obstetrics and Gynecology·Nancy S GreenUNKNOWN March Of Dimes Scientific Advisory Committee On Prematurity
Nov 4, 2005·The New England Journal of Medicine·Sharon L Hillier
Nov 4, 2005·The New England Journal of Medicine·David N FredricksJeanne M Marrazzo

❮ Previous
Next ❯

Citations

Jul 26, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Kristina M Adams Waldorf, Ryan M McAdams
Jan 11, 2014·Journal of Perinatology : Official Journal of the California Perinatal Association·J Romano-KeelerJ L Maron
Jun 6, 2013·Clinical & Developmental Immunology·Emilie Tourneur, Cecilia Chassin
Mar 19, 2014·Pediatric Research·Matthew S PayneAlan H Jobe
Sep 19, 2014·Science Translational Medicine·Harvey J Kliman
Sep 19, 2014·Science Translational Medicine·Kjersti M Aagaard
May 20, 2015·Oxford Medical Case Reports·Utpala DeviJagadish Mahanta
Jun 25, 2014·Trends in Molecular Medicine·Yuliya E BorreJohn F Cryan
Jan 5, 2011·Infectious Diseases in Obstetrics and Gynecology·Xia ZhouLarry J Forney
Jan 10, 2012·Annual Review of Food Science and Technology·Petra A M J ScholtensJan Knol
Jun 6, 2014·The Proceedings of the Nutrition Society·Lotta NylundWillem M de Vos
Jan 31, 2015·NeoReviews·Bin CaoIndira U Mysorekar
Nov 14, 2014·Science Translational Medicine·Craig E RubensCourtney Gravett
Apr 2, 2011·Journal of Perinatology : Official Journal of the California Perinatal Association·R Murgas Torrazza, J Neu
Dec 14, 2011·Journal of Perinatology : Official Journal of the California Perinatal Association·A S WeintraubL Rand
Mar 27, 2010·BMC Pregnancy and Childbirth·Maureen KelleyUNKNOWN GAPPS Review Group
Mar 30, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Chong Jai KimJung-Sun Kim
May 21, 2010·Journal of Perinatal Medicine·Daniel B DiGiulioDavid A Relman
May 21, 2010·Journal of Perinatal Medicine·Daniel B DiGiulioDavid A Relman
Mar 19, 2011·Current Opinion in Pediatrics·Flavia Indrio, Josef Neu
May 5, 2011·The Journal of Perinatal & Neonatal Nursing·Katherine E Gregory
May 13, 2011·American Journal of Respiratory Cell and Molecular Biology·Steffen KunzmannBoris W Kramer
Nov 1, 2012·Current Opinion in Pediatrics·Juliette C MadanMargaret R Karagas
Feb 26, 2013·Italian Journal of Pediatrics·Antonio Di MauroFlavia Indrio
Sep 6, 2013·Environmental Health Perspectives·Lindsey Konkel
Mar 13, 2014·PloS One·Alexandria N ArdissoneJosef Neu
Feb 22, 2012·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Daniel N FrankEdward N Janoff
Sep 22, 2011·Journal of Perinatal Medicine·Catherine Ford, Mehmet R Genç
Jan 11, 2013·BMC Genomics·Michael D HarwichKimberly K Jefferson
Feb 2, 2013·Current Opinion in Rheumatology·Amy D ProalTrevor G Marshall
Jun 28, 2011·Neonatology·Boris W Kramer
Apr 12, 2013·Immunologic Research·Amy D ProalInge A Lindseth
Nov 26, 2014·Current Pediatrics Reports·Josef Neu
Jan 11, 2013·Archives of Disease in Childhood. Fetal and Neonatal Edition·Eoin BarrettCatherine Stanton
May 7, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Molly J StoutIndira U Mysorekar

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

âRâ
âEpiâ
âSurvivalâ

Related Concepts

Related Feeds

Archaeogenetics

Recent advances in genomic sequencing has led to the discovery of new strains of Archaea and shed light on their evolutionary history. Discover the latest research on Archaeogenetics here.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.

© 2022 Meta ULC. All rights reserved