Microbial production of conjugated gamma-linolenic acid from gamma-linolenic acid by Lactobacillus plantarum AKU 1009a

Journal of Applied Microbiology
S KishinoS Shimizu

Abstract

Optimal production conditions of conjugated gamma-linolenic acid (CGLA) from gamma-linolenic acid using washed cells of Lactobacillus plantarum AKU 1009a as catalysts were investigated. Washed cells of Lact. plantarum AKU 1009a exhibiting a high level of CGLA productivity were obtained by cultivation in a nutrient medium supplemented with 0.03% (w/v) alpha-linolenic acid as an inducer. Under the optimal reaction conditions with 13 mg ml(-1)gamma-linolenic acid as a substrate in 5 -ml reaction volume, the washed cells [32% (wet cells, w/v) corresponding to 46 mg ml(-1) dry cells] as the catalysts produced 8.8 mg CGLA per millilitre reaction mixture (68% molar yield) in 27 h. The produced CGLA was a mixture of two isomers, i.e., cis-6,cis-9,trans-11-octadecatrienoic acid (CGLA1, 40% of total CGLA) and cis-6,trans-9,trans-11-octadecatrienoic acid (CGLA2, 60% of total CGLA), and accounted for 66% of total fatty acid obtained. The CGLA produced was obtained as free fatty acids adsorbed mostly on the surface of the cells of Lact. plantarum AKU1009a. The practical process of CGLA production from gamma-linolenic acid using washed cells of Lact. plantarum AKU 1009a was successfully established. We presented the first example of microbia...Continue Reading

References

Jun 20, 2001·Progress in Lipid Research·M W ParizaM E Cook
Mar 7, 2003·Bioscience, Biotechnology, and Biochemistry·Shigenobu KishinoSakayu Shimizu
Oct 4, 2005·Journal of Bioscience and Bioengineering·Koji Nagao, Teruyoshi Yanagita
Nov 29, 2005·Journal of Bioscience and Bioengineering·Jun OgawaSakayu Shimizu
Mar 25, 2006·Bioscience, Biotechnology, and Biochemistry·Jun OgawaSakayu Shimizu
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Oct 17, 2007·Journal of Oleo Science·Sayantani Dasgupta, Dipak Kumar Bhattacharyya
Jan 8, 2008·Progress in Lipid Research·Koji Nagao, Teruyoshi Yanagita

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Citations

Oct 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shigenobu KishinoJun Ogawa
Mar 13, 2016·Lipids·Alan A HennessyCatherine Stanton
Apr 7, 2005·Current Opinion in Chemical Biology·Takeru IshigeSakayu Shimizu
Jul 9, 2014·Journal of Lipid Research·Haruko SakuramaJun Ogawa
Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Lara GorissenKatleen Raes
Jul 2, 2019·Critical Reviews in Food Science and Nutrition·Marco GobbettiCarlo Giuseppe Rizzello
Aug 31, 2018·Microbiology and Molecular Biology Reviews : MMBR·Ana S SalsinhaLuis M Rodríguez-Alcalá

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