Microbial production of medium-chain-length 3-hydroxyalkanoic acids by recombinant Pseudomonas putida KT2442 harboring genes fadL, fadD and phaZ

FEMS Microbiology Letters
Mei-Qing YuanGuo-Qiang Chen

Abstract

Monomers of microbial polyhydroxyalkanoates, mainly 3-hydroxyhexanoic acid (3HHx) and 3-hydroxyoctanoic acid (3HO), were produced by overexpressing polyhydroxyalkanoates depolymerase gene phaZ, together with putative long-chain fatty acid transport protein fadL of Pseudomonas putida KT2442 and acyl-CoA synthetase (fadD) of Escherichia coli MG1655 in P. putida KT2442. FadL(Pp), which is responsible for free fatty acid transportation from the extracellular environment to the cytoplasm, and FadD(Ec), which activates fatty acid to acyl-CoA, jointly reinforce the fatty acid beta-oxidation pathway. Pseudomonas putida KT2442 (pYZPst01) harboring polyhydroxyalkanoates depolymerase gene phaZ of Pseudomonas stutzeri 1317 produced 1.37 g L(-1) extracellular 3HHx and 3HO in shake flask studies after 48 h in the presence of sodium octanoate as a sole carbon source, while P. putida KT2442 (pYZPst06) harboring phaZ(Pst), fadD(Ec) and fadL(Pp) achieved 2.32 g L(-1) extracellular 3HHx and 3HO monomer production under the same conditions. In a 48-h fed-batch fermentation process conducted in a 6-L fermentor with 3 L sodium octanoate mineral medium, 5.8 g L(-1) extracellular 3HHx and 3HO were obtained in the fermentation broth. This is the first ...Continue Reading

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Jul 5, 2007·Applied Microbiology and Biotechnology·Qian LiuGuo-Qiang Chen

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Citations

Apr 16, 2010·Applied Microbiology and Biotechnology·Qun RenManfred Zinn
Sep 28, 2015·Trends in Biotechnology·Guo-Qiang Chen, Ivan Hajnal
Jun 24, 2015·Applied Microbiology and Biotechnology·Anita Loeschcke, Stephan Thies
Jun 28, 2011·Current Opinion in Biotechnology·Xue GaoGuo-Qiang Chen
Apr 30, 2016·International Journal of Biological Macromolecules·Anbreen AnjumShazia Tabasum
Sep 25, 2016·Microbiological Research·Justyna Możejko-Ciesielska, Robert Kiewisz
Jul 23, 2009·Chemical Society Reviews·Guo-Qiang Chen
Jun 17, 2021·Essays in Biochemistry·Dillirani NagarajanJo-Shu Chang
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Dattatray K BedadeRichard A Gross

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