Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid

Applied Microbiology and Biotechnology
R MüllerE Leistner

Abstract

When transformed with a recombinant vector carrying the ubiC gene (encoding chorismate pyruvate-lyase, EC 4.1.3.27) the triple mutant (Phe-, Trp-, Tyr-) Klebsiella pneumoniae 62-1 excretes 4-hydroxybenzoic acid instead of chorismic acid. The recombinant strain can be used to produce in high yield specifically ring-labelled 4-hydroxybenzoic acid from isotopically labelled glucose.

References

Nov 1, 1986·Journal of Natural Products·H G FlossJ M Beale
Feb 20, 1995·Biotechnology and Bioengineering·K Schmidt, E Leistner

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Citations

May 15, 2012·Journal of Biotechnology·Jens O KrömerCristian Varela
Dec 15, 2016·Frontiers in Bioengineering and Biotechnology·Shiqin YuJens O Krömer
Feb 28, 2019·Microbial Cell Factories·Damla HuccetogullariSang Yup Lee
Jun 28, 2019·Frontiers in Bioengineering and Biotechnology·Christoph LenzenNick Wierckx

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