Microbial Succession during a Field Evaluation of Phenol and Toluene as the Primary Substrates for Trichloroethene Cometabolism.

Applied and Environmental Microbiology
M R FriesJ M Tiedje

Abstract

Microbial community composition and succession were studied in an aquifer that was amended with phenol, toluene, and chlorinated aliphatic hydrocarbons to evaluate the effectiveness of these aromatic substrates for stimulating trichloroethene (TCE) bioremediation. Samples were taken after the previous year's field studies, which used phenol as the primary substrate, and after three successive monthly treatments of phenol plus 1,1-dichloroethene (1,1-DCE) plus TCE, phenol plus TCE, and toluene plus TCE. Dominant eubacteria in the community were assessed after each of the four treatments by characterizing isolates from the most dilute most-probable-number tubes and by extracting DNA from aquifer samples. The succession of dominant phenol- and toluene-degrading strains was evaluated by genomic fingerprinting, cellular fatty acid methyl ester (FAME) analysis, and amplified ribosomal DNA restriction analysis (ARDRA). 1,1-DCE was found to drastically reduce microbial growth and species richness, which corresponded to the reduction in bioremediation effectiveness noted previously for this treatment (G. D. Hopkins and P. L. McCarty, Environ. Sci. Technol. 29:1628-1637, 1995). Only a few gram-positive isolates could be obtained after tr...Continue Reading

References

Jul 1, 1988·Applied and Environmental Microbiology·L P Wackett, D T Gibson
May 1, 1987·Applied and Environmental Microbiology·M J NelsonP H Pritchard
Jan 1, 1985·Applied and Environmental Microbiology·J T Wilson, B H Wilson
Apr 1, 1983·Applied and Environmental Microbiology·E J Bouwer, P L McCarty
Aug 1, 1994·Applied and Environmental Microbiology·M R FriesJ M Tiedje
Oct 1, 1989·Applied and Environmental Microbiology·L P Wackett, S R Householder
Aug 1, 1987·Environmental Science & Technology·T M VogelP L McCarty

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Citations

Jun 1, 2000·Applied and Environmental Microbiology·A S Whiteley, M J Bailey
Feb 20, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jizhong ZhouAdam P Arkin
Feb 26, 2016·Scientific Reports·Shuo JiaoGehong Wei
Jul 30, 2004·Journal of Applied Microbiology·R J Grant, W B Betts
Sep 27, 2002·Applied and Environmental Microbiology·R E ParalesL P Wackett
Aug 27, 2005·Canadian Journal of Microbiology·J A HumphriesC G Johnston
Jul 31, 2004·Annual Review of Phytopathology·Mark Mazzola
Jul 31, 1999·Applied and Environmental Microbiology·A FernándezJ Tiedje

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