Microbial Synthesis of Non-Natural Anthraquinone Glucosides Displaying Superior Antiproliferative Properties

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Trang Thi Huyen NguyenJae Kyung Sohng

Abstract

Anthraquinones, naturally occurring bioactive compounds, have been reported to exhibit various biological activities, including anti-inflammatory, antiviral, antimicrobial, and anticancer effects. In this study, we biotransformed three selected anthraquinones into their novel O-glucoside derivatives, expressing a versatile glycosyltransferase (YjiC) from Bacillus licheniformis DSM 13 in Escherichia coli. Anthraflavic acid, alizarin, and 2-amino-3-hydroxyanthraquinone were exogenously fed to recombinant E. coli as substrate for biotransformation. The products anthraflavic acid-O-glucoside, alizarin 2-O-β-d-glucoside, and 2-amino-3-O-glucosyl anthraquinone produced in the culture broths were characterized by various chromatographic and spectroscopic analyses. The comparative anti-proliferative assay against various cancer cells (gastric cancer-AGS, uterine cervical cancer-HeLa, and liver cancer-HepG2) were remarkable, since the synthesized glucoside compounds showed more than 60% of cell growth inhibition at concentrations ranging from ~50 μM to 100 μM. Importantly, one of the synthesized glucoside derivatives, alizarin 2-O-glucoside inhibited more than 90% of cell growth in all the cancer cell lines tested.

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Citations

Feb 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Roberto Fabiani
Jun 27, 2020·Journal of Industrial Microbiology & Biotechnology·Trang Thi Huyen NguyenJae Kyung Sohng
Nov 6, 2020·International Journal of Biological Macromolecules·Bing LiuRui Bao
Aug 24, 2021·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Amina M DirirLina F Yousef

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Methods Mentioned

BETA
glycosylation
NMR
glycosylating

Software Mentioned

MestReNova

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