Microbial synthesis of polyhydroxybutyrate from glycerol: gluconeogenesis, molecular weight and material properties of biopolyester

Biotechnology and Bioengineering
Nuttapol Tanadchangsaeng, Jian Yu

Abstract

Glycerol is considered as an ideal feedstock for producing bioplastics via bacterial fermentation due to its ubiquity, low price, and high degree of reduction substrate. In this work, we study the yield and cause of limitation in poly(3-hydroxybutyrate) (PHB) production from glycerol. Compared to glucose-based PHB production, PHB produced by Cupriavidus necator grown on glycerol has a low productivity (0.92 g PHB/L/h) with a comparably low maximum specific growth rate of 0.11 h(-1) . We found that C. necator can synthesize glucose from glycerol and that the lithotrophical utilization of glycerol (non-fermentative substrate) or gluconeogenesis is an essential metabolic pathway for biosynthesis of cellular components. Here, we show that gluconeogenesis affects the reduction of cell mass, the productivity of biopolymer product, and the molecular chain size of intracellular PHB synthesized from glycerol by C. necator. We use NMR spectroscopy to show that the isolated PHB is capped by glycerol. We then characterized the physical properties of the isolated glycerol-based PHB with differential scanning calorimetry and tensile tests. We found that although the final molecular weight of the glycerol-based PHB is lower than those of gluc...Continue Reading

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Citations

Dec 18, 2013·Applied Microbiology and Biotechnology·Sebastian L RiedelChristopher J Brigham
Jun 1, 2014·Applied Microbiology and Biotechnology·Toshiaki FukuiSatoshi Nakamura
Sep 4, 2015·Critical Reviews in Biotechnology·Elena VolodinaAlexander Steinbüchel
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Jul 1, 2014·Current Opinion in Biotechnology·Ying WangGuo-Qiang Chen
Nov 5, 2016·Applied Biochemistry and Biotechnology·Li-Jung HsiaoSi-Yu Li
Jun 28, 2018·Journal of Industrial Microbiology & Biotechnology·Xiangbin ChenGuo-Qiang Chen
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Mar 13, 2020·Biotechnology Journal·Yu Jung SohnSi Jae Park
Apr 4, 2015·International Journal of Biological Macromolecules·Prasun KumarVipin C Kalia

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