Microbially competent 3D skin: a test system that reveals insight into host-microbe interactions and their potential toxicological impact.

Archives of Toxicology
Lisa LemoineTewes Tralau

Abstract

The skin`s microbiome is predominantly commensalic, harbouring a metabolic potential far exceeding that of its host. While there is clear evidence that bacteria-dependent metabolism of pollutants modulates the toxicity for the host there is still a lack of models for investigating causality of microbiome-associated pathophysiology or toxicity. We now report on a biologically characterised microbial-skin tissue co-culture that allows studying microbe-host interactions for extended periods of time in situ. The system is based on a commercially available 3D skin model. In a proof-of-concept, this model was colonised with single and mixed cultures of two selected skin commensals. Two different methods were used to quantify the bacteria on the surface of the skin models. While Micrococcus luteus established a stable microbial-skin tissue co-culture, Pseudomonas oleovorans maintained slow continuous growth over the 8-day cultivation period. A detailed skin transcriptome analysis showed bacterial colonisation leading to up to 3318 significant changes. Additionally, FACS, ELISA and Western blot analyses were carried out to analyse secretion of cytokines and growth factors. Changes found in colonised skin varied depending on the bacteri...Continue Reading

References

Oct 19, 1999·Human & Experimental Toxicology·T PlatzekW Baltes
Apr 2, 2002·The Journal of Investigative Dermatology. Symposium Proceedings·K ChillerG J Murakawa
Nov 15, 2002·Proceedings of the National Academy of Sciences of the United States of America·Thaddeus S StappenbeckJeffrey I Gordon
Feb 28, 2003·Science's STKE : Signal Transduction Knowledge Environment·Aisling Dunne, Luke A J O'Neill
Jun 7, 2003·Science·Gregory M Barton, Ruslan Medzhitov
Feb 25, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Diego PreciadoFrank Ondrey
Jul 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ruth E LeyJeffrey I Gordon
May 23, 2006·Pharmaceutical Research·Enam KhalilGerald B Kasting
Oct 19, 2007·Nature·Peter J TurnbaughJeffrey I Gordon
Nov 27, 2007·FEMS Immunology and Medical Microbiology·Mihai G NeteaBart Jan Kullberg
Dec 18, 2007·FEMS Microbiology Letters·Diana B HollandKeith T Holland
Jan 15, 2008·Immunology Letters·Anja S Tessarz, Adelheid Cerwenka
May 27, 2008·Genome Research·Elizabeth A GriceJulia A Segre
Jun 11, 2008·Immunology·Wendy W C van MarenGosse J Adema
Aug 7, 2008·International Journal of Pharmaceutics·Tiago SousaAbdul W Basit
Nov 26, 2008·Gastroenterology·Andrew S Neish
Feb 17, 2009·Trends in Immunology·Yuping Lai, Richard L Gallo
May 30, 2009·Science·Elizabeth A GriceJulia A Segre
Jul 15, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heimo LaglerSylvia Knapp
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·T Andrew ClaytonJeremy K Nicholson
Sep 5, 2009·Journal of Medical Microbiology·Robin L StingleyCarl E Cerniglia
Dec 2, 2009·Journal of Bacteriology·Michael YoungCharles L Greenblatt
Mar 31, 2010·Toxicology in Vitro : an International Journal Published in Association with BIBRA·T HuM J Aardema
Nov 5, 2010·The Journal of Investigative Dermatology·Ines WankeBirgit Schittek
Mar 3, 2011·MBio·Sandrine P ClausJeremy K Nicholson
Oct 25, 2011·Cytokine & Growth Factor Reviews·Gloria Lopez-Castejon, David Brough
Feb 1, 2012·Antimicrobial Agents and Chemotherapy·Anna de BreijPeter H Nibbering
Mar 24, 2012·PloS One·Khoa Nguyen DoAlexandr Parlesak
Apr 3, 2012·Nature·Christina E ZielinskiFederica Sallusto
May 23, 2012·Current Opinion in Immunology·Katherine M Nutsch, Chyi-Song Hsieh
Jun 26, 2012·Nature Reviews. Immunology·Richard L Gallo, Lora V Hooper

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
PCA
ELISA
profiler
biopsies
FACS

Software Mentioned

MatTek
GraphPad Prism
Transcriptome Analysis Console
Ingenuity Pathway Analysis (
7500 Fast SDS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.