Microbiome analysis of healthy and diseased sponges Lubomirskia baicalensis by using cell cultures of primmorphs

PeerJ
Lubov ChernogorSergei Belikov

Abstract

Endemic sponges (Demosponges, Lubomirskiidae) dominate the fauna of the littoral zone of Lake Baikal. These freshwater sponges live in symbiosis with diverse eukaryotes and prokaryotes, including chlorophyll-containing microalgae. Within the last 5 years, the incidence of sponge disease and mortality events in Lake Baikal has increased. The etiology and ecology of these events remain unknown, in part because of the lack of models to study sponge-microbe interactions. In this work, we tested the use of primmorph cell cultures of Lubomirskia baicalensis as a tool for investigating the microbiomes of sponges. We infected primmorphs, cultured in vitro, with samples from diseased sponges and observed, by microscopy, disease symptoms, including loss of green symbionts, associated with mass die-off events. Subsequent sequencing of 16S rRNA gene fragments revealed that the microbiome community of healthy sponge and primmorphs formed a group separate from the community of diseased sponges and infected primmorphs. This confirms the suitability of the primmorph cell culture as a model sponge system. We also discovered mass mortality of green symbionts (Chlorophyta) was associated with a shift in the microbial communities of sponges/primmo...Continue Reading

References

Jul 21, 1999·Journal of Biotechnology·M H MunroA R Duckworth
Feb 10, 2004·Applied and Environmental Microbiology·Nicole S WebsterAndrew P Negri
Dec 8, 2005·Applied and Environmental Microbiology·Catherine Lozupone, Rob Knight
Sep 8, 2006·Diseases of Aquatic Organisms·J B OlsonM Slattery
May 17, 2007·Environmental Microbiology·Nicole S Webster
Jun 8, 2007·Microbiology and Molecular Biology Reviews : MMBR·Michael W TaylorMichael Wagner
Dec 15, 2007·Science·O Hoegh-GuldbergM E Hatziolos
Jul 14, 2010·Applied and Environmental Microbiology·Heidi M LuterNicole S Webster
Dec 1, 2010·FEMS Microbiology Ecology·Aaron W Miller, Laurie L Richardson
Dec 1, 2010·Marine Drugs·Sunil SagarKenneth P Minneman
Jan 12, 2011·Marine Biotechnology·Lubov I ChernogorAlexander V Ereskovsky
Mar 30, 2011·Environmental Microbiology·Nicole S Webster, Michael W Taylor
Jun 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Lu FanTorsten Thomas
Aug 14, 2012·Applied and Environmental Microbiology·Patrick M ErwinXavier Turon
Jan 4, 2013·PloS One·Rachel SimisterNicole Webster
Jun 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·A Murat ErenJessica L Mark Welch
Jul 12, 2014·The ISME Journal·Mikhail TikhonovNed S Wingreen
May 30, 2015·Recent Patents on Drug Delivery & Formulation·Abolfazl Shakeri, Amirhossein Sahebkar
Aug 5, 2015·PloS One·Sarah A Gignoux-Wolfsohn, Steven V Vollmer
Sep 4, 2015·Bioscience, Biotechnology, and Biochemistry·Hitoshi NakayamaShunji Hattori
May 24, 2016·Nature Methods·Benjamin J CallahanSusan P Holmes
Jun 17, 2016·Nature Communications·Torsten ThomasNicole S Webster
Mar 16, 2017·BMC Evolutionary Biology·Hanna KinnulaLotta-Riina Sundberg
Aug 11, 2017·PLoS Pathogens·Lance B PriceCindy M Liu
Oct 13, 2017·GigaScience·Lucas Moitinho-SilvaTorsten Thomas
Nov 2, 2017·Nature·Luke R ThompsonUNKNOWN Earth Microbiome Project Consortium
Jul 26, 2019·Nature Biotechnology·Evan BolyenJ Gregory Caporaso

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Citations

Jun 27, 2020·Microbiology Resource Announcements·Ivan S PetrushinLubov I Chernogor
May 6, 2021·In Vitro Cellular & Developmental Biology. Animal·Yuefan SongQi Yang

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Datasets Mentioned

BETA
PRJNA480187

Methods Mentioned

BETA
mechanical dissociation
scanning electron microscopy
PCR
Fluorescence
Fluorescence microscopy

Software Mentioned

QIIME
Principal Coordinates Analysis ( PCoA )
QIIME2
tidyr R package
ggplot2 R package
DADA2
PERMANOVA

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