Microbiome analysis of the saliva and midgut from partially or fully engorged female adult Dermacentor silvarum ticks in China

Experimental & Applied Acarology
De-Yong DuanTian-Yin Cheng

Abstract

Dermacentor silvarum is widely distributed in northern China and transmits several pathogens that cause diseases in humans and domestic animals. We analysed the comprehensive bacterial community of the saliva and midgut from partially and fully engorged female adult D. silvarum. Dermacentor silvarum samples were collected from Guyuan, China. Bacterial DNA was extracted from the saliva and midgut contents of partially or fully engorged female adult D. silvarum. Sequencing of the V3-V4 hypervariable regions of the 16S rRNA genes was performed using the IonS5TMXL platform. The bacterial diversity in saliva was higher than in the midgut. The bacterial diversity of saliva from fully engorged ticks was greater than in partially engorged tick saliva. The bacterial diversity in midguts from partially engorged ticks was greater than in fully engorged tick midguts. Proteobacteria was the most dominant bacterial phylum in all of the samples. Twenty-nine bacterial genera were detected in all of the samples. Rickettsia, Anaplasma, and Stenotrophomonas were the main genera. The symbionts Coxiella, Arsenophonus, and Wolbachia were also detected in all of the samples. Eight bacterial species were identified in all of the experimental samples. ...Continue Reading

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Datasets Mentioned

BETA
PRJNA495117
KP258209.1

Methods Mentioned

BETA
dissecting
electrophoresis
PCR

Software Mentioned

R
QIIME
Uparse
MUSCLE
Primer Premier
Cutadapt

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