Microbiome and Culture Based Analysis of Chronic Rhinosinusitis Compared to Healthy Sinus Mucosa

Frontiers in Microbiology
Kerstin KoellerBernhard Olzowy

Abstract

The role of bacteria in chronic rhinosinusitis (CRS) is still not well understood. Whole microbiome analysis adds new aspects to our current understanding that is mainly based on isolated bacteria. It is still unclear how the results of microbiome analysis and the classical culture based approaches interrelate. To address this, middle meatus swabs and tissue samples were obtained during sinus surgery in 5 patients with CRS with nasal polyps (CRSwNP), 5 patients with diffuse CRS without nasal polyps (CRSsNP), 5 patients with unilateral purulent maxillary CRS (upm CRS) and 3 patients with healthy sinus mucosa. Swabs were cultured, and associated bacteria were identified. Additionally, parts of each tissue sample also underwent culture approaches, and in parallel DNA was extracted for 16S rRNA gene amplicon-based microbiome analysis. From tissue samples 4.2 ± 1.2 distinct species per patient were cultured, from swabs 5.4 ± 1.6. The most frequently cultured species from the swabs were Propionibacterium acnes, Staphylococcus epidermidis, Corynebacterium spp. and Staphylococcus aureus. The 16S-RNA gene analysis revealed no clear differentiation of the bacterial community of healthy compared to CRS samples of unilateral purulent maxil...Continue Reading

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Citations

Apr 20, 2020·International Forum of Allergy & Rhinology·Stephen M HumphriesDavid A Lynch
Dec 14, 2018·Current Opinion in Allergy and Clinical Immunology·Jivianne T LeeVijay Ramakrishnan
Jan 29, 2021·Microbial Biotechnology·Ilke De BoeckSarah Lebeer
Mar 28, 2021·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Joanna SzaleniecMaciej Szaleniec
Apr 25, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Junwon Yang, Jongsik Chun
Jul 3, 2021·Allergy, Asthma & Immunology Research·Yi Sook KimHyun Woo Shin

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Datasets Mentioned

BETA
PRJEB23675

Methods Mentioned

BETA
biopsies
PCR
pharmacotherapy
biopsy

Software Mentioned

LEfSe
SILVAngs
BLAST
PAST
Explicet
SILVA Incremental Aligner ( SINA
SILVA
fastq
CoNet

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