Microbiome Profiling Reveals Gut Dysbiosis in the Metabotropic Glutamate Receptor 5 Knockout Mouse Model of Schizophrenia

Frontiers in Cell and Developmental Biology
Carolina GubertAnthony J Hannan

Abstract

Schizophrenia (SZ) is a psychiatric disorder that constitutes one of the top 10 global causes of disability. More recently, a potential pathogenic role for the gut microbial community (microbiota) has been highlighted, with numerous studies describing dysregulated microbial profiles in SZ patients when compared to healthy controls. However, no animal model of SZ has previously recapitulated the gut dysbiosis observed clinically. Since the metabotropic glutamate receptor 5 (mGlu5) knockout mice provide a preclinical model of SZ with strong face and predictive validity, in the present study we performed gut microbiome profiling of mGlu5 knockout (KO) and wild-type (WT) mice by 16S rRNA sequencing of bacterial genomic DNA from fecal samples, analyzing bacterial diversity and taxonomic composition, as well as gastrointestinal parameters as indicators of gut function. We found a significant genotype difference in microbial beta diversity. Analysis of composition of microbiomes (ANCOM) models were performed to evaluate microbiota compositions, which identified a decreased relative abundance of the Erysipelotrichaceae family and Allobaculum genus in this mouse model of SZ. We also identified a signature of bacteria discriminating betw...Continue Reading

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Citations

May 6, 2021·Biomolecules·Vivek Kumar SharmaMohamed M Abdel-Daim
Jun 23, 2021·Current Opinion in Psychiatry·Jerzy Samochowiec, Błażej Misiak
Aug 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Katarzyna SocałaPiotr Wlaź

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Datasets Mentioned

BETA
PRJNA659149

Methods Mentioned

BETA
biopsy
amplicon sequencing

Software Mentioned

Graph ® Pad Prism
PERMANOVA
SparCC
DA
mixOmics
Qiita
UniFrac
Sparse Correlations for Compositional ( SparCC )
qgraph
sPLS

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