Microbiome-related metabolite changes in gut tissue, cecum content and feces of rats treated with antibiotics

Toxicology and Applied Pharmacology
C BehrB van Ravenzwaay

Abstract

The metabolic functionality of the gut microbiota contributes to the metabolism and well-being of its host, although detailed insight in the microbiota's metabolism is lacking. Omics technologies could facilitate unraveling metabolism by the gut microbiota. In this study, we performed metabolite profiling of different matrices of the gut, after antibiotic treatment of rats in order to evaluate metabolite changes observed at different dose levels and in different sexes, and to identify the best tissue matrix for further investigations regarding an assessment of metabolic effects of new compounds with antibiotic activity. Three different antibiotics (vancomycin, streptomycin and roxithromycin) were administered orally to rats for 28 days according to the OECD 407 guideline with a subsequent metabolic profiling in feces, cecum content and gut tissue (jejunum, ileum, cecum, colon and rectum). The data were analyzed in the MetaMap®Tox database. Treatment-related effects could be observed in the metabolite profile of feces and cecum content, but not of the different gut tissues. The metabolite profile showed compound specific effects on the microbiome. In line with the activity spectra of the antibiotics tested, vancomycin showed the...Continue Reading

Citations

Feb 27, 2020·Cell Host & Microbe·Sidhartha R SinhaAida Habtezion
May 2, 2021·Journal of Hazardous Materials·Tharushi Prabha KeerthisingheMingliang Fang
Nov 21, 2020·Journal of Agricultural and Food Chemistry·Chen LiuIvonne M C M Rietjens
Dec 3, 2021·Journal of Agricultural and Food Chemistry·Qianrui WangIvonne M C M Rietjens

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