Microbiome within Primary Tumor Tissue from Renal Cell Carcinoma May Be Associated with PD-L1 Expression of the Venous Tumor Thrombus

Advances in Urology
Michael LissDharam Kaushik

Abstract

To perform a proof of concept microbiome evaluation and PD-L1 expression profiling in clear-cell renal cell carcinoma (cc-RCC) with associated tumor thrombus (TT). After IRB approval, six patients underwent radical nephrectomy (RN) with venous tumor thrombectomy (VTT). We collected fresh tissue specimens from normal adjacent, tumor, and thrombus tissues. We utilized RNA sequencing to obtain PD-L1 expression profiles and perform microbiome analysis. Statistical assessment was performed using Student's t-test, chi-square, and spearman rank correlations using SPSS v25. We noted the tumor thrombus to be mostly devoid of diverse microbiota. A large proportion of Staphylococcus epidermidus was detected and unknown if this is a surgical or postsurgical contaminant; however, it was noted more in the thrombus than other tissues. Microbiome diversity profiles were most abundant in the primary tumor compared to the thrombus or normal adjacent tissue. Differential expression of PD-L1 was examined in the tumor thrombus to the normal background tissue and noted three of the six subjects had a threshold above 2-fold. These three similar subjects had foreign microbiota that are typical residents of the oral microbiome. Renal tumors have more d...Continue Reading

References

Jan 13, 2012·Urologic Oncology·Adam C ReeseMaxwell V Meng
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Jul 15, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sumanta K PalPaul Frankel
Apr 2, 2016·Therapeutic Advances in Urology·Leonardo D BorregalesJose A Karam
Oct 4, 2016·Nature Reviews. Nephrology·Yasser Riazalhosseini, Mark Lathrop
Jan 6, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Apr 4, 2017·Annals of Oncology : Official Journal of the European Society for Medical Oncology·N ChaputF Carbonnel
Aug 2, 2017·ESMO Open·Alejo Rodriguez-VidaMichiel H Strijbos
Apr 11, 2018·Cancer Cell·Vancheswaran GopalakrishnanJennifer A Wargo
Apr 17, 2018·Cell·Samra TurajlicUNKNOWN TRACERx Renal Consortium
Aug 16, 2018·Scientific Reports·Viljemka Bučević PopovićJanoš Terzić
Aug 21, 2018·International Journal of Cancer. Journal International Du Cancer·Khalid SaeedPäivi Östling
Dec 7, 2018·Cancers·Estíbaliz López-Fernández, José I López

❮ Previous
Next ❯

Citations

Nov 22, 2020·Journal of Cancer Research and Clinical Oncology·Junpeng WangTianzhong Yan

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
RNA-seq
biopsies

Software Mentioned

Pathoscope
Bowtie2
count
Trimmomatic
HTSeq
TopHat2

Related Concepts

Related Feeds

Adenocarcinoma, Clear Cell

Clear Cell Adenocarcinoma is a tumor that arises in the female genital tract and is characterized by cells that appear clear under the microscope. Discover the latest research here.