Microbiomes of China's Space Station During Assembly, Integration, and Test Operations

Microbial Ecology
Ying ZhangYu-Lin Deng

Abstract

Sufficient evidence indicates that orbiting space stations contain diverse microbial populations, which may threaten astronaut health and equipment reliability. Understanding the composition of microbial communities in space stations will facilitate further development of targeted biological safety prevention and maintenance practices. Therefore, this study systematically investigated the microbial community of China's Space Station (CSS). Air and surface samples from 46 sites on the CSS and Assembly Integration and Test (AIT) center were collected, from which 40 bacteria strains were isolated and identified. Most isolates were cold- and desiccation-resistant and adapted to oligotrophic conditions. Bacillus was the dominant bacterial genus detected by both cultivation-based and Illumina MiSeq amplicon sequencing methods. Microbial contamination on the CSS was correlated with encapsulation staff activities. Analysis by spread plate and qPCR revealed that the CSS surface contained 2.24 × 103-5.47 × 103 CFU/100 cm2 culturable bacteria and 9.32 × 105-5.64 × 106 16S rRNA gene copies/100cm2; BacLight™ analysis revealed that the viable/total bacterial cell ratio was 1.98-13.28%. This is the first study to provide important systematic ...Continue Reading

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Citations

Sep 25, 2020·International Journal of Molecular Sciences·Irina BuchovecPranciškus Vitta
Feb 20, 2021·Microbiology Resource Announcements·Velislava IlievaMichael C Macey

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Datasets Mentioned

BETA
SRP105019

Methods Mentioned

BETA
PMA
PCR
PCA
the
ISS

Software Mentioned

SILVA
Mothur
QIIME
CLUSTAL W
R
FLASH
vegan
PAST
PERMANOVA
InteractiVenn

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