Microbiomic comparison of the intestine of the earthworm Eisenia fetida fed ergovaline.

Current Microbiology
Rogan M RattrayA M Craig

Abstract

Tall fescue toxicosis and ergot alkaloids cost U.S. livestock producers approximately one billion dollars in annual livestock production loss annually. Ergovaline (EV) is the tall fescue alkaloid primarily responsible for clinical disease in livestock. Since native ruminal microorganisms have not been attributed to the detoxification of EV, finding detoxifying microbes from other environments is desirable. One possible source for potential microorganisms that can degrade EV is the anaerobic gut of the earthworm, Eisenia fetida. This study describes a comparative microbial analysis of earthworm digestive tracts receiving 10,000 ppb EV (E+ treatment) when compared with a control treatment with no detectable amounts of EV (E- treatment). An HPLC assay determined a 25% loss of EV from the E+ treatment was microbial in nature. A community microbiomic approach of constructing 16S-rRNA gene clone libraries was used to compare the microbes affected by the two treatments. RDPII tools such as Classifier and Libcompare were used in the analysis of 16S sequences. DOTUR analysis was used to examine the richness and diversity of the two microbial populations in these experiments. The results indicate there are few significant differences in ...Continue Reading

References

Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
May 1, 1995·Journal of Animal Science·B T LarsonJ T Turner
Jul 1, 1994·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·A M CràigR E Welty
Jun 8, 2001·Environmental Toxicology and Chemistry·S S HumphriesA J Stewart
Aug 3, 2001·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J M SchnitziusA M Craig
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·D G PanaccioneC L Schardl
Aug 7, 2003·Applied and Environmental Microbiology·Jonathan L SebatRonald L Crawford
Sep 24, 2004·Current Biology : CB·Henrik GlennerEske Willerslev
Mar 5, 2005·Applied and Environmental Microbiology·Patrick D Schloss, Jo Handelsman
Aug 27, 2005·Canadian Journal of Microbiology·S L Lodge-IveyA M Craig
Sep 30, 2005·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J M DuringerA M Craig
Jan 5, 2006·Applied and Environmental Microbiology·Seana K Davidson, David A Stahl
Mar 1, 1995·Applied and Environmental Microbiology·G R Karsten, H L Drake
Dec 13, 2007·Nucleic Acids Research·Dennis A BensonDavid L Wheeler

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