Microbiota dynamics and diversity at different stages of industrial processing of cocoa beans into cocoa powder.

Applied and Environmental Microbiology
Lídia J R LimaM J Rob Nout

Abstract

We sampled a cocoa powder production line to investigate the impact of processing on the microbial community size and diversity at different stages. Classical microbiological methods were combined with 16S rRNA gene PCR-denaturing gradient gel electrophoresis, coupled with clone library construction, to analyze the samples. Aerobic thermoresistant spores (ThrS) (100°C; 10 min) were also isolated and characterized (identity, genetic diversity, and spore heat resistance), in view of their relevance to the quality of downstream heat-treated cocoa-flavored drinks. In the nibs (broken, shelled cocoa beans), average levels of total aerobic microorganisms (TAM) (4.4 to 5.6 log CFU/g) and aerobic total spores (TS) (80°C; 10 min; 4.3 to 5.5 log CFU/g) were significantly reduced (P < 0.05) as a result of alkalizing, while fungi (4.2 to 4.4 log CFU/g) and Enterobacteriaceae (1.7 to 2.8 log CFU/g) were inactivated to levels below the detection limit, remaining undetectable throughout processing. Roasting further decreased the levels of TAM and TS, but they increased slightly during subsequent processing. Molecular characterization of bacterial communities based on enriched cocoa samples revealed a predominance of members of the Bacillaceae...Continue Reading

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Citations

Jun 25, 2013·International Journal of Food Microbiology·Luca CocolinKalliopi Rantsiou
Jul 6, 2014·International Journal of Food Microbiology·Cecilie L MarvigDennis S Nielsen
Dec 15, 2015·International Journal of Food Microbiology·D Sylvain DabadéHeidy M W den Besten
Sep 4, 2013·Phytotherapy Research : PTR·Grace FarhatE A S Al-Dujaili
Aug 27, 2013·International Journal of Food Microbiology·Genia LückingMonika Ehling-Schulz
Apr 28, 2020·Journal of Food Protection·Robyn T EijlanderMarjon H J Wells-Bennik
Sep 10, 2018·Food Research International·Dorota ŻyżelewiczMilena Kaczmarska
Jun 10, 2021·Applied and Environmental Microbiology·O G G Almeida, E C P De Martinis

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