Microbiota in a cooling-lubrication circuit and an option for controlling triethanolamine biodegradation

Biofouling
Thomas KlammsteinerMaraike Probst

Abstract

Cooling and lubrication agents like triethanolamine (TEA) are essential for many purposes in industry. Due to biodegradation, they need continuous replacement, and byproducts of degradation may be toxic. This study investigates an industrial (1,200 m³) cooling-lubrication circuit (CLC) that has been in operation for 20 years and is supposedly in an ecological equilibrium, thus offering a unique habitat. Next-generation (Illumina Miseq 16S rRNA amplicon) sequencing was used to profile the CLC-based microbiota and relate it to TEA and bicine dynamics at the sampling sites, influent, machine rooms, biofilms and effluent. Pseudomonas pseudoalcaligenes dominated the effluent and influent sites, while Alcaligenes faecalis dominated biofilms, and both species were identified as the major TEA degrading bacteria. It was shown that a 15 min heat treatment at 50°C was able to slow down the growth of both species, a promising option to control TEA degradation at large scale.

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Citations

Feb 8, 2019·Applied Microbiology and Biotechnology·Martín Vargas-SuárezHerminia Loza-Tavera

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Methods Mentioned

BETA
PCR
thermal treatment

Software Mentioned

R Development Core Team
disana
vegan
mothur
Chromaeleon Data System
R
msa
labdsv
BLAST®

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