Microbiota-Mediated Modulation of Organophosphate Insecticide Toxicity by Species-Dependent Interactions with Lactobacilli in a Drosophila melanogaster Insect Model

Applied and Environmental Microbiology
Brendan A DaisleyGregor Reid

Abstract

Despite the benefits to the global food supply and agricultural economies, pesticides are believed to pose a threat to the health of both humans and wildlife. Chlorpyrifos (CP), a commonly used organophosphate insecticide, has poor target specificity and causes acute neurotoxicity in a wide range of species via the suppression of acetylcholinesterase. This effect is exacerbated 10- to 100-fold by chlorpyrifos oxon (CPO), a principal metabolite of CP. Since many animal-associated symbiont microorganisms are known to hydrolyze CP into CPO, we used a Drosophila melanogaster insect model to investigate the hypothesis that indigenous and probiotic bacteria could affect CP metabolism and toxicity. Antibiotic-treated and germfree D. melanogaster insects lived significantly longer than their conventionally reared counterparts when exposed to 10 μM CP. Drosophila melanogaster gut-derived Lactobacillus plantarum, but not Acetobacterindonesiensis, was shown to metabolize CP. Liquid chromatography tandem-mass spectrometry confirmed that the L. plantarum isolate preferentially metabolized CP into CPO when grown in CP-spiked culture medium. Further experiments showed that monoassociating germfree D. melanogaster with the L. plantarum isolate...Continue Reading

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Citations

Oct 31, 2019·The ISME Journal·Brendan A DaisleyGregor Reid
Jan 23, 2019·World Journal of Microbiology & Biotechnology·Sen XieYongqi Shao
Oct 27, 2020·Frontiers in Microbiology·Alison G Blanton, Brittany F Peterson
Apr 11, 2021·European Journal of Nutrition·Narimane DjekkounHafida Khorsi-Cauet
Jun 3, 2021·International Journal of Environmental Research and Public Health·Federica GiambòConcettina Fenga
Sep 21, 2021·Frontiers in Microbiology·Ana Cuesta-MatéMichael Poulsen

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