Microbiota modification by probiotic supplementation reduces colitis associated colon cancer in mice

World Journal of Gastroenterology : WJG
Maria Carolina S MendesJosé Barreto C Carvalheira

Abstract

To investigate the effect of probiotic supplementation during the development of an experimental model of colitis associated colon cancer (CAC). C57BL/6 mice received an intraperitoneal injection of azoxymethane (10 mg/kg), followed by three cycles of sodium dextran sulphate diluted in water (5% w/v). Probiotic group received daily a mixture of Lactobacillus acidophilus, Lactobacillus rhamnosus and Bifidobacterium bifidum. Microbiota composition was assessed by 16S rRNA Illumina HiSeq sequencing. Colon samples were collected for histological analysis. Tumor cytokines was assessed by Real Time-PCR (Polymerase Chain Reaction); and serum cytokines by Multiplex assay. All tests were two-sided. The level of significance was set at P < 0.05. Graphs were generated and statistical analysis performed using the software GraphPad Prism 5.0. The project was approved by the institutional review board committee. At day 60 after azoxymethane injection, the mean number of tumours in the probiotic group was 40% lower than that in the control group, and the probiotic group exhibited tumours of smaller size (< 2 mm) (P < 0.05). There was no difference in richness and diversity between groups. However, there was a significant difference in beta di...Continue Reading

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Methods Mentioned

BETA
PCR
protein extraction

Software Mentioned

USEARCH
UPARSE
R package Phyloseq
Permanova
vegan
GraphPad Prism

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