Microbisporicin gene cluster reveals unusual features of lantibiotic biosynthesis in actinomycetes.

Proceedings of the National Academy of Sciences of the United States of America
Lucy C Foulston, Mervyn Bibb

Abstract

Lantibiotics are ribosomally synthesized, posttranslationally modified peptide antibiotics. The biosynthetic gene cluster for microbisporicin, a potent lantibiotic produced by the actinomycete Microbispora corallina containing chlorinated tryptophan and dihydroxyproline residues, was identified by genome scanning and isolated from an M. corallina cosmid library. Heterologous expression in Nonomuraea sp. ATCC 39727 confirmed that all of the genes required for microbisporicin biosynthesis were present in the cluster. Deletion, in M. corallina, of the gene (mibA) predicted to encode the prepropeptide abolished microbisporicin production. Further deletion analysis revealed insights into the biosynthesis of this unusual and potentially clinically useful lantibiotic, shedding light on mechanisms of regulation and self-resistance. In particular, we report an example of the involvement of a tryptophan halogenase in the modification of a ribosomally synthesized peptide and the pathway-specific regulation of an antibiotic biosynthetic gene cluster by an extracytoplasmic function sigma factor-anti-sigma factor complex.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 18, 1998·Chemistry & Biology·A M van WageningenP J Solenberg
Nov 11, 1999·International Journal of Systematic Bacteriology·Y NakajimaT Kudo
Jun 1, 2000·Applied and Environmental Microbiology·K AltenaG Bierbaum
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertolt GustKeith F Chater
Mar 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·D A WiddickM J Bibb
Jul 3, 2003·Chemistry & Biology·Margherita SosioStefano Donadio
Feb 11, 2005·Chemical Reviews·Champak ChatterjeeWilfred A van der Donk
Jun 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Koji YanaiMervyn Bibb
May 8, 2007·Nucleic Acids Research·Lukas KällErik L L Sonnhammer
Mar 14, 2008·Current Protein & Peptide Science·Lorraine A DraperPaul D Cotter
Oct 23, 2008·Chemistry & Biology·Lisa E CooperWilfred A van der Donk
Jan 9, 2010·The Journal of Antibiotics·Giorgia Letizia MarconeFabrizio Beltrametti
Mar 24, 2010·Antimicrobial Agents and Chemotherapy·Giorgia Letizia MarconeFlavia Marinelli

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Citations

Apr 25, 2012·Applied Microbiology and Biotechnology·Steven BoakesJesús Cortés
Aug 19, 2010·Journal of Industrial Microbiology & Biotechnology·Giorgia Letizia MarconeFabrizio Beltrametti
Mar 23, 2011·Biotechnology Letters·Jia Zeng, Jixun Zhan
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Qi ZhangWilfred A van der Donk
Jun 19, 2013·Proceedings of the National Academy of Sciences of the United States of America·Emma J Sherwood, Mervyn J Bibb
Jul 12, 2011·Applied and Environmental Microbiology·Ju-Hoon LeeDaniel J O'Sullivan
Apr 12, 2011·Journal of Bacteriology·Lucy Foulston, Mervyn Bibb
Aug 10, 2011·Evidence-based Complementary and Alternative Medicine : ECAM·Xin-Qing Zhao
Feb 5, 2013·BMC Microbiology·Alicia CampionR Paul Ross
Mar 4, 2014·PloS One·Kai BlinTilmann Weber
Jan 16, 2014·Journal of Natural Products·Sonia I MaffioliStefano Donadio
Oct 8, 2013·Journal of Industrial Microbiology & Biotechnology·Juan Pablo Gomez-Escribano, Mervyn J Bibb
Mar 13, 2012·Annual Review of Biochemistry·Patrick J Knerr, Wilfred A van der Donk
Aug 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Qi ZhangWilfred A van der Donk
Sep 14, 2015·Microbiology·Carolin HöflerThorsten Mascher
Oct 31, 2015·Journal of Industrial Microbiology & Biotechnology·Sonia I MaffioliStefano Donadio
Jun 12, 2012·Expert Opinion on Drug Discovery·Manuel Montalbán-LópezOscar P Kuipers
Oct 9, 2015·Applied Microbiology and Biotechnology·Jun-Sheng ChenDai-Jie Chen
Oct 26, 2010·Trends in Biotechnology·Srdjan JoveticJohannes Tramper
Apr 27, 2011·Biotechnology and Bioengineering·Amitha P ReddyJean S VanderGheynst
Nov 26, 2010·Molecular Microbiology·Des FieldR Paul Ross
Jun 27, 2014·Molecular Microbiology·Stefanie MakJustin R Nodwell
May 6, 2015·Molecular Microbiology·Lorena T Fernández-MartínezMervyn J Bibb
Nov 22, 2013·Biochemical Society Transactions·Mervyn J Bibb
Sep 9, 2015·BioMed Research International·Lucia CarranoFlavia Marinelli
Apr 19, 2016·Marine Drugs·Juan Pablo Gomez-EscribanoMervyn J Bibb
Aug 13, 2013·Chemistry & Biology·Arianna TocchettiStefano Donadio
Feb 26, 2013·Current Opinion in Chemical Biology·Duncan R M SmithRebecca J M Goss
Jul 1, 2014·Journal of Biotechnology·Y Martínez-BurgoP Liras
Jan 21, 2015·International Journal of Medical Microbiology : IJMM·Evi StegmannRoberta Pozzi
Jan 13, 2015·ACS Chemical Biology·Sonia I MaffioliStefano Donadio
Sep 4, 2016·FEMS Microbiology Reviews·Manuel Montalbán-LópezOscar P Kuipers
Dec 19, 2015·PLoS Pathogens·Dirk-Jan Scheffers, Menno B Tol
Jan 7, 2017·Chemical Reviews·Mireille MoutiezMuriel Gondry

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