Microbubble-mediated ultrasound promotes accumulation of bone marrow mesenchymal stem cell to the prostate for treating chronic bacterial prostatitis in rats

Scientific Reports
Shan-hong YiLongkun Li

Abstract

Chronic bacterial prostatitis (CBP) is an intractable disease. Although bone marrow mesenchymal stem cells (BMMSCs) are able to regulate inflammation in CBP, the effect of microbubble-mediated ultrasound- induced accumulation of BMMSCs on CBP remains unclear. To address this gap, a model of CBP was established in SD rats, which were then treated with BMMSCs alone (BMMSC group), BMMSCs with ultrasound (ultrasound group), BMMSCs with microbubble-mediated ultrasound (MMUS group) and compared with a healthy control group. A therapeutic-ultrasound apparatus was used to treat the prostate in the presence of circulating microbubbles and BMMSCs. The BMMSC distribution was assessed with in vivo imaging, and the prostate structure with light microscopy. Real-time quantitative RT-PCR, ELISA, and immunohistochemistry were used to assess the expressions of TNF-α and IL-1β. More BMMSCs were found in the prostate in the MMUS group than in the CBP, ultrasound, and BMMSC groups. Inflammatory cell infiltration, fibrous tissue hyperplasia, and tumor-like epithelial proliferation were significantly reduced in the MMUS group, as were the mRNA and protein expressions of TNF-α and IL-1β. Microbubble-mediated ultrasound-induced accumulation of BMMSCs ...Continue Reading

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Citations

Feb 8, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Pasquale MarrazzoLaura Bonsi
Mar 12, 2020·Stem Cells Translational Medicine·Daniel D LiuAvnesh S Thakor
May 20, 2020·Frontiers in Pharmacology·Situ XiongBin Fu
Nov 9, 2019·Ultrasound in Medicine & Biology·Kirby R LattweinKlazina Kooiman
Mar 14, 2020·Ultrasound in Medicine & Biology·Klazina KooimanChristy K Holland

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Methods Mentioned

BETA
flow cytometry
fluorescence
transfection
light microscopy
ELISA
PCR

Software Mentioned

ImagePro
Plus

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