Microcalorimetric studies of the biological effects of Ho(III) on Halobacterium halobium R1

Biological Trace Element Research
Liu PengShen Ping

Abstract

The biological effect of Ho3+ on Halobacterium halobium R1 growth was analyzed using the microcalorimetric method. Using the LKB-2277 Bioactivity Monitor with the ampoule method at 37 degrees C, the thermogenic curves of the growth of H. halobium R1 were obtained. Then, the maximum power (P (m)) and the growth rate constants (k) were determined, and the values of P (m) and k were linked to the concentration of Ho3+. In all, the addition of Ho3+ cause a decrease in the maximum heat production and growth rate constants. To confirm the results, the shapes of H. halobium R1 cell addition with Ho3+ using a transmission electron microscope (TEM) were observed. According to the thermogenic curves and TEM photos of H. halobium R1 under different conditions, it is clear that the metabolic mechanism of H. halobium R1 growth has been changed with the addition of Ho3+.

References

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Nov 22, 2000·Journal of Biochemical and Biophysical Methods·Z RumingQ Songsheng
Dec 9, 2003·Journal of Inorganic Biochemistry·Peng LiuSongsheng Qu
Jun 3, 2005·Biological Trace Element Research·Liu PengQu Songsheng

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