Microcalorimetric studies of the interaction mechanisms between proteins and Q-sepharose at pH near the isoelectric point (pI) effects of NaCl concentration, pH value, and temperature

Journal of Chromatography. a
F Y LinS Yamamoto

Abstract

This study examined the interaction mechanisms of beta-lactoglobulins A and B (Lg A, Lg B) with an anion exchanger, Q-Sepharose at pH near the isoelectric point at which the proteins are expected to be electrically neutralized under various NaCl concentrations and temperatures by the equilibrium binding analysis and the adsorption enthalpy directly measured by isothermal titration calorimetry. The data evaluated from isotherms fitted by the Langmuirean model reveal that the addition of NaCl considerably reduced the binding affinities and capacities of both the proteins with Q-Sepharose at pH 5.2, indicating that electrostatic forces are dominant during the adsorption. However, the hydrophobic interaction seems to be involved in adsorption as well at a higher NaCl concentration, and the adsorption enthalpies confirm this suggestion. In addition, the effects of temperature on the equilibrium binding behaviors for Lg A or Lg B with Q-Sepharose were found to be salt concentration-dependent, probably due to their different binding mechanisms at 0.03 M and 0.3 M NaCl. Where, at 0.3 M NaCl, the hydrophobic interaction plays a more pronounced role. This implication was again supported by the adsorption enthalpies. The presented data pr...Continue Reading

Citations

Apr 16, 2002·Journal of Molecular Recognition : JMR·Fu-Yung LinMilton T W Hearn
Jun 14, 2003·Journal of Colloid and Interface Science·Hsiang-Ming HuangRuoh-Chyu Ruaan
Aug 29, 2012·Journal of the American Chemical Society·Shih-Hui LeeKenneth J Shea
Feb 5, 2016·Journal of Molecular Recognition : JMR·Chih-Pei LinMilton T W Hearn
Sep 7, 2018·Journal of Cellular Physiology·Maryam Rahmati, Masoud Mozafari
Feb 9, 2019·Preparative Biochemistry & Biotechnology·Agnes RodlerAlois Jungbauer
Feb 10, 2009·Biotechnology and Bioengineering·Syed M Saufi, Conan J Fee
Nov 11, 2009·Biotechnology Journal·L Selva RoselinWen-Yih Chen
Aug 8, 2006·Biotechnology Journal·Klaus Graumann, Andreas Premstaller
Apr 25, 2019·Biotechnology Progress·Swarnim RanjanSteven M Cramer
Nov 1, 2012·Journal of Separation Science·Rainer Hahn
Jan 28, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Leilei LianGuangming Liu
Apr 11, 2018·Journal of Chromatography. a·Beate Beyer, Alois Jungbauer
Jun 8, 2021·Colloids and Surfaces. B, Biointerfaces·Joao Carlos Simoes-CardosoShuichi Yamamoto
May 14, 2004·Journal of Chromatography. a·Jessica M Phillips, Neville G Pinto
Oct 14, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Kartik SrinivasanSteven M Cramer
Mar 13, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Joao C Simoes-CardosoShuichi Yamamoto
Aug 15, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Kai ZhengYisheng Xu
Apr 18, 2009·Colloids and Surfaces. B, Biointerfaces·Jalil BadraghiAli Akbar Moosavi-Movahedi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.