Microcalorimetric study of adsorption of human monoclonal antibodies on cation exchange chromatographic materials

Journal of Chromatography. a
Michael DieterleHans Hasse

Abstract

Adsorption of two human monoclonal antibodies on two different strong cation exchange resins is studied by isothermal titration microcalorimetry and independent adsorption isotherm measurements. The pH value is varied between 4.5 and 7.0, using different buffer systems, the temperature is always 25 degrees C. The adsorption isotherm data is fitted using two different Langmuir type models. Combining the calorimetric and the adsorption data, the specific enthalpy of adsorption of the protein Deltah(p)(ads) is determined. At pH values near 7.0, where the antibodies are only weakly charged, the adsorption is exothermal. At small loadings the absolute number of Deltah(p)(ads) is then large and almost constant but it significantly decreases at higher loadings. This shows that the arrangement of antibody molecules on the absorber material depends on the loading and is less favourable at higher loadings. Despite the high positive charge of the antibody at pH values of about 5 the value of Deltah(p)(ads) is almost zero along the entire isotherm. Furthermore, at pH 4.5 even endothermal effects are observed, although high binding capacities are found. At these conditions the adsorption process seems to be strongly influenced by the ions b...Continue Reading

References

Feb 3, 2004·Journal of Chromatography. a·Deborah K Follman, Robert L Fahrner
Apr 19, 2007·Journal of Chromatography. a·Florian Dismer, Jürgen Hubbuch

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Citations

Feb 5, 2016·Journal of Molecular Recognition : JMR·Chih-Pei LinMilton T W Hearn
Jan 19, 2012·Journal of Chromatography. a·Line Naomi LundPhaedria M St Hilaire
Jun 22, 2011·Journal of Chromatography. a·Tim BlaschkeHans Hasse
Apr 4, 2009·Journal of Chromatography. a·Rafael A LiraMaria C H da Silva
Mar 17, 2009·Journal of Hazardous Materials·Wei-Jun KongYong-Shen Ren
Nov 1, 2012·Journal of Separation Science·Rainer Hahn
Mar 10, 2010·Journal of Molecular Recognition : JMR·Robert J FalconerBrett M Collins
Nov 11, 2009·Biotechnology Journal·L Selva RoselinWen-Yih Chen
Jul 27, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Albert WernerHans Hasse
Feb 9, 2019·Preparative Biochemistry & Biotechnology·Agnes RodlerAlois Jungbauer
Apr 11, 2018·Journal of Chromatography. a·Beate Beyer, Alois Jungbauer
Jun 8, 2021·Colloids and Surfaces. B, Biointerfaces·Joao Carlos Simoes-CardosoShuichi Yamamoto
Mar 13, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Joao C Simoes-CardosoShuichi Yamamoto

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