Jan 1, 1976

Microcalorimetric study of the anaerobic growth of Escherichia coli: measurements of the affinity of whole cells for various energy substrates

Journal of Bacteriology
A Belaich, J P Belaich

Abstract

Microcalorimetry has been used to determine the affinity of whole cells of Escherichia coli for glucose, galactose, fructose, and lactose. Anaerobic growth thermograms were analyzed, and the Km and Vmax values for these energy substrates were measured at pH 7.8. Results obtained with this technique using various organisms growing anaerobically on different sugars are compared. This comparison shows that in practically all cases the cellular rate of catabolic activity is a hyperbolic function of the energy substrate concentrations at low sugar concentrations. In some cases this technique also allows determination of kinetics at high sugar concentrations.

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Mentioned in this Paper

Anhydrous lactose
Galactose Measurement
Alkalescens-Dispar Group
Fructose
Catabolic Process
Glucose, (beta-D)-Isomer
Galactose
Calorimetry
Anaerobiosis
Levulosa Ife

About this Paper

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