Microcalorimetric study on the effect of Ce3+ on Halobacterium halobium R1

Biological Trace Element Research
Yi LiuPing Shen

Abstract

A microcalorimeric technique was used to evaluate the influence of rare earths Ce3+ on Halobacterium halobium R1 growth. By means of TAM air Thermal Activity Monitor, the thermogenic curves of H. halobium R1 growth were obtained. To analyze the results, the growth rate constant k and IC50 were calculated, indicating that the values of k are linked to the concentration of Ce3+. The growth rate constant k of H. halobium R1 decreased gradually in the low concentration; thus, rare earths restrained the growth of H. halobium R1. On the contrary, as the concentration of Ce3+ became higher, the value of k for H. halobium R1 increased gradually, which showed Ce3+ stimulated the growth of H. halobium R1. When the concentration of rare earths became much higher, the value of k for H. halobium R1 also decreased, and the growth of H. halobium R1 was restrained totally in the end. By using transmission electron microscopy (TEM), it was observed that the transforming of H. halobium R1 in the different concentrations of Ce3+ confirmed the results derived from microcalorimetry. According to the thermogenic curves and TEM photos of H. halobium R1 under various conditions, it showed that there was some special effect about the interaction betwee...Continue Reading

References

Nov 22, 2000·Journal of Biochemical and Biophysical Methods·Z RumingQ Songsheng
Mar 1, 2005·Journal of Biochemical and Biophysical Methods·Yang YangSongsheng Qu

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