PMID: 16512409Mar 4, 2006Paper

Microcantilevers modified by horseradish peroxidase intercalated nano-assembly for hydrogen peroxide detection

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Xiaodong YanHai Feng Ji

Abstract

Detection of hydrogen peroxide is of industry interest and of biological importance. Here we report a new approach to hydrogen peroxide measurement using multilayer modified microcantilevers. Through a layer-by-layer nanoassembly technique, horseradish peroxidase was intercalated into a nanoscale multilayer assembly on one surface of microcantilevers. These enzyme-functionalized microcantilevers deflected in response to hydrogen peroxide concentrations in the nanomolar level. The magnitudes of bending were proportional to the concentrations of hydrogen peroxide. Furthermore, our study also showed that microcantilever technique may be used as a novel, more sensitive tool for the study of the conformational or structural changes of enzymes or proteins on surfaces.

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Oct 1, 2005·Analytical Chemistry·Xiaodong YanHai-Feng Ji

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Citations

Dec 25, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Hongyan GaoHai-Feng Ji
Jul 4, 2006·Trends in Biotechnology·Barry J RyanCiarán O'Fágáin
May 16, 2018·Dalton Transactions : an International Journal of Inorganic Chemistry·Debashis BasuThomas B Rauchfuss
Mar 28, 2008·The Analyst·Hai-Feng JiMarvin K Schulte
Mar 12, 2011·The Analyst·Koutilya R BuchapudiThomas Thundat

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