Microcarrier culture: A different approach to granulosa cell cultivation.

Cytotechnology
S StoklosowaJ Galas

Abstract

Granulosa cells (GC) are steroid secreting and hormone responsive cells synthesizing increased amounts of progesterone (P(4)) under the influence of gonadotropins. Up to now these cells have been cultivated as monolayers. However, in this system, cells dedifferentiate early and cease to respond to stimulation. GC are a good model for studying hormonal regulation of ovarian cell function. In this study, microcarrier culture was tested for the first time to see whether it would be a good system in which GC would grow and give a stronger responsivity to gonadotropins. Cells were grown in stationary culture on microcarriers Cytodex 3 (C3) coated with collagen and on pure gelatin beads. Only C3 cultures were successful. Cells proliferated better on C3 microcarriers and on day 4 of culture secreted more P(4) under the influence of FSH and LH than comparative monolayer cultures.

References

Apr 1, 1977·Metabolism: Clinical and Experimental·C P Channing, A Tsafriri
Jan 1, 1979·Biochimie·G Fayet, S Hovsepian
Oct 13, 1992·Annals of the New York Academy of Sciences·B Griffiths
Jun 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·G R Klinefelter, L L Ewing
Jan 1, 1972·In Vitro·T V Fischer, R H Kahn
Jun 1, 1982·Biology of Reproduction·S StoklosowaC P Channing
Jan 1, 1994·Cytotechnology·J B Griffiths, A J Racher

❮ Previous
Next ❯

Citations

Aug 26, 1998·Acta histochemica·I KmicikiewiczB Bilińska

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Physical Chemistry Chemical Physics : PCCP
Naima A HutterRainer Jordan
In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association
M A HadleyM Dym
© 2022 Meta ULC. All rights reserved