Microcavity arrays as an in vitro model system of the bone marrow niche for hematopoietic stem cells

Cell and Tissue Research
Patrick WuchterEric Gottwald

Abstract

In previous studies human mesenchymal stromal cells (MSCs) maintained the "stemness" of human hematopoietic progenitor cells (HPCs) through direct cell-cell contact in two-dimensional co-culture systems. We establish a three-dimensional (3D) co-culture system based on a custom-made chip, the 3(D)-KITChip, as an in vitro model system of the human hematopoietic stem cell niche. This array of up to 625 microcavities, with 300 μm size in each orientation, was inserted into a microfluidic bioreactor. The microcavities of the 3(D)-KITChip were inoculated with human bone marrow MSCs together with umbilical cord blood HPCs. MSCs used the microcavities as a scaffold to build a complex 3D mesh. HPCs were distributed three-dimensionally inside this MSC network and formed ß-catenin- and N-cadherin-based intercellular junctions to the surrounding MSCs. Using RT(2)-PCR and western blots, we demonstrate that a proportion of HPCs maintained the expression of CD34 throughout a culture period of 14 days. In colony-forming unit assays, the hematopoietic stem cell plasticity remained similar after 14 days of bioreactor co-culture, whereas monolayer co-cultures showed increasing signs of HPC differentiation and loss of stemness. These data support ...Continue Reading

References

Jun 1, 1977·Journal of Cellular Physiology·T M DexterL G Lajtha
Apr 1, 1986·Journal of Cellular Physiology·S TsaiD G Nathan
Mar 10, 2000·Science·T ChengD T Scadden
Mar 15, 2000·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S SienaC Carlo-Stella
Feb 16, 2006·IEE Proceedings. Nanobiotechnology·S GiselbrechtK F Weibezahn
Jun 1, 2007·Lab on a Chip·Eric GottwaldKarl-Friedrich Weibezahn
Dec 11, 2008·Journal of Visualized Experiments : JoVE·Stefan GiselbrechtKarl-Friedrich Weibezahn
Dec 11, 2008·Journal of Visualized Experiments : JoVE·Eric GottwaldKarl-Friedrich Weibezahn
May 13, 2009·Journal of Cellular and Molecular Medicine·Thomas WalendaWolfgang Wagner
Aug 13, 2010·Nature·Simón Méndez-FerrerPaul S Frenette
Mar 15, 2011·Advanced Materials·Roman TruckenmüllerClemens van Blitterswijk
Mar 16, 2011·The Journal of Experimental Medicine·Armin Ehninger, Andreas Trumpp
Nov 20, 2012·Advances in Experimental Medicine and Biology·Rupert Handgretinger, Selim Kuçi
Nov 2, 2013·World Journal of Stem Cells·Meimei LiuShang-Tian Yang

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Citations

Feb 16, 2019·Advanced Materials·Pinak SamalStefan Giselbrecht
Apr 4, 2019·Advanced Healthcare Materials·Maaike V J BrahamJacqueline Alblas
Jun 5, 2019·Bioengineering·Cordula NiesEric Gottwald
May 22, 2019·International Journal of Molecular Sciences·Hyeon Joo YooLeopold Sellner
Dec 12, 2020·PloS One·Gabriel Santos RosalemMaria Gabriela Reis Carvalho
Nov 6, 2020·Developmental Biology·Nicola GrittiVikas Trivedi
May 18, 2021·Frontiers in Cell and Developmental Biology·Thomas BessyDiana Passaro
Jul 3, 2021·Chemical Reviews·Farhad SoheilmoghaddamJustin Cooper-White
Sep 28, 2021·The Journal of Cell Biology·Thomas BessyManuel Théry

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