Microcell-mediated chromosome transfer identifies EPB41L3 as a functional suppressor of epithelial ovarian cancers.

Neoplasia : an International Journal for Oncology Research
Dimitra DafouSimon A Gayther

Abstract

We used a functional complementation approach to identify tumor-suppressor genes and putative therapeutic targets for ovarian cancer. Microcell-mediated transfer of chromosome 18 in the ovarian cancer cell line TOV21G induced in vitro and in vivo neoplastic suppression. Gene expression microarray profiling in TOV21G(+18) hybrids identified 14 candidate genes on chromosome 18 that were significantly overexpressed and therefore associated with neoplastic suppression. Further analysis of messenger RNA and protein expression for these genes in additional ovarian cancer cell lines indicated that EPB41L3 (erythrocyte membrane protein band 4.1-like 3, alternative names DAL-1 and 4.1B) was a candidate ovarian cancer-suppressor gene. Immunoblot analysis showed that EPB41L3 was activated in TOV21G(+18) hybrids, expressed in normal ovarian epithelial cell lines, but was absent in 15 (78%) of 19 ovarian cancer cell lines. Using immunohistochemistry, 66% of 794 invasive ovarian tumors showed no EPB41L3 expression compared with only 24% of benign ovarian tumors and 0% of normal ovarian epithelial tissues. EPB41L3 was extensively methylated in ovarian cancer cell lines and primary ovarian tumors compared with normal tissues (P = .00004), sugg...Continue Reading

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Citations

Apr 2, 2015·Epigenetics : Official Journal of the DNA Methylation Society·Christian HuismanMarianne G Rots
Dec 10, 2013·The Journal of Allergy and Clinical Immunology·Salome ScholtensH Marike Boezen
Apr 2, 2013·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·David Guerrero-SetasRosa Guarch
Aug 7, 2018·Epigenetics : Official Journal of the DNA Methylation Society·Wina VerlaatG Bea A Wisman
Nov 14, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Fang ZhaoHonglin Liu
Jul 12, 2012·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yajie ZhangHongyan Wang
Sep 4, 2014·Cellular and Molecular Life Sciences : CMLS·Zi WangXiuli An
Sep 24, 2019·International Journal of Oncology·Xiaofeng YuanZhiwei Liu
Sep 8, 2019·Cell Communication and Signaling : CCS·Fumin XuePengyuan Zheng

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