Microcell-mediated cotransfer of genes specifying methotrexate resistance, emetine sensitivity, and chromate sensitivity with Chinese hamster chromosome 2.

Molecular and Cellular Biology
R G WortonW F Flintoff

Abstract

Many selectable mutants of somatic Chinese hamster cells have been described, but very few of the mutations have been mapped to specific chromosomes. We have utilized the microcell-mediated gene transfer technique to establish the location of three selectable genetic markers on chromosome 2 of Chinese hamster. Microcells were prepared from the methotrexate-resistant MtxRIII line of Flintoff et al. (Somatic Cell Genet. 2:245-261, 1976) and fused to wild-type CHO cells, and microcell hybrids (transferants) were selected in medium containing methotrexate. All transferants were karyotyped and found to contain a marker chromosome from the donor MtxRIII line. This marker chromosome, called 2p-, consisted of a chromosome 2 with a reduced short arm resulting from a reciprocal translocation between 2p and 5q. In experiments utilizing emetine-resistant (Emtr) or chromate-resistant (Chrr) recipient cells it was found that the emt+ and chr+ wild-type genes were cotransferred with the 2p- chromosomes. Karyotype analysis of several transferants with rearranged or broken 2p- markers allowed regional localization of the emt and chr loci to the proximal third of the long arm and localization of the gene or genes conferring methotrexate resistan...Continue Reading

References

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Citations

May 1, 1986·Experimental Cell Research·D Hernandez-VerdunM Bouteille
Nov 1, 1986·Somatic Cell and Molecular Genetics·V L Funanage, T T Myoda
Nov 1, 1986·Somatic Cell and Molecular Genetics·G UrlaubJ Hamlin
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Aug 1, 1986·Molecular and Cellular Biology·D D RhoadsD J Roufa
Oct 1, 1984·Molecular and Cellular Biology·V L FunanageH R Cowell

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