Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1.

The Journal of Biological Chemistry
Xingzhi XuDavid F Stern

Abstract

Microcephalin (MCPH1) is the first gene identified among at least six loci that contribute to the autosomal recessive disease, primary microcephaly. MCPH1, like NFBD1/MDC1, 53BP1, and BRCA1, encodes a protein with twin carboxyl-terminal BRCT domains (PTCB). Here, we report that Mcph1 forms ionizing radiation-induced foci. Down-regulation of Mcph1, like other PTCBs, by siRNA, impairs ionizing radiation-induced intra-S-phase and G(2)/M checkpoints. Inhibition of the expression of Mcph1 decreases both protein and transcript levels of endogenous Brca1 but not exogenous Brca1. Mcph1 inhibition also decreases both endogenous and heterologous Chk1 transcripts and protein. We conclude that Mcph1 is involved in DNA damage-induced cellular responses, and we propose that regulation of Brca1 and/or Chk1 by Mcph1 may contribute to these cellular responses.

References

Apr 21, 2001·Molecular and Cellular Biology·B XuM B Kastan
Jun 8, 2001·Molecular and Cellular Biology·H Zhao, H Piwnica-Worms
Feb 12, 2002·Nature Genetics·Ronit I YardenLawrence C Brody
Oct 2, 2002·Nature Genetics·Jacquelyn BondC Geoffrey Woods
Oct 5, 2002·Science·Bin WangStephen J Elledge
Dec 21, 2002·The Journal of Biological Chemistry·Lyuben TsvetkovDavid F Stern
Dec 25, 2002·The Journal of Biological Chemistry·Xingzhi Xu, David F Stern
Jan 29, 2003·The Journal of Biological Chemistry·Aimin Peng, Phang-Lang Chen
Jan 31, 2003·Nature·Christopher J Bakkenist, Michael B Kastan
Feb 28, 2003·Nature·Michal GoldbergStephen P Jackson
Feb 28, 2003·Nature·Grant S StewartStephen J Elledge
Apr 5, 2003·The Journal of Biological Chemistry·Zhan XiaoHaiying Zhang
Jul 3, 2003·Cell·Shiaw Yih Lin, Stephen J Elledge
Oct 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xingzhi Xu, David F Stern
Oct 25, 2003·Science·Isaac A MankeMichael B Yaffe
Oct 25, 2003·Science·Xiaochun YuJunjie Chen
Oct 28, 2003·The Journal of Biological Chemistry·Maria RodriguezZhou Songyang
Nov 19, 2003·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Mohnish Suri

❮ Previous
Next ❯

Citations

Jul 16, 2010·Breast Cancer Research and Treatment·Julie RichardsonSandra M Bell
Sep 2, 2011·Journal of Molecular Neuroscience : MN·Orly ReinerGabi Gerlitz
Jun 20, 2006·Nature Cell Biology·Gemma K AldertonMark O'Driscoll
Jun 16, 2009·Nature Cell Biology·Guang PengShiaw-Yih Lin
Mar 29, 2005·Nature Genetics·Jacquelyn BondC Geoffrey Woods
Jul 28, 2010·Oncogene·J A L BrownC G Morrison
Aug 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Namit SinghGeorges Mer
Apr 26, 2011·The Journal of Biological Chemistry·Justin W LeungJunjie Chen
Aug 23, 2008·The Journal of Biological Chemistry·Jamie L WoodJunjie Chen
Oct 2, 2009·Carcinogenesis·William K Kaufmann
Jul 31, 2008·Human Molecular Genetics·Tom StiffPenny A Jeggo
Nov 14, 2006·Current Opinion in Pediatrics·Gewei Lian, Volney Sheen
Aug 2, 2006·Molecular and Cellular Biology·Shizhou LiuJiri Lukas
Sep 25, 2007·Annual Review of Genomics and Human Genetics·Peter J McKinnon, Keith W Caldecott
Jun 25, 2009·Cancer Research·Xianglin WuFergus J Couch
Jul 9, 2011·The Journal of Clinical Investigation·Sandrine PassemardVincent Lelièvre
Sep 15, 2011·BMC Biochemistry·Dale CorkeryJoseph Torchia
Oct 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shiaw-Yih LinStephen J Elledge
Feb 25, 2014·European Journal of Cell Biology·Thejaswini Venkatesh, Padmanaban S Suresh
Sep 14, 2011·The Journal of Cell Biology·Daisuke YamashitaTatsuya Hirano
Jul 25, 2015·International Journal of Cancer. Journal International Du Cancer·Giulia PartipiloAnita Mangia
Nov 26, 2009·Progress in Neurobiology·Angela M KaindlPierre Gressens
Sep 26, 2009·Seminars in Pediatric Neurology·Ganeshwaran H Mochida
Oct 14, 2008·Trends in Neurosciences·Eric J VallenderBruce T Lahn
Nov 13, 2007·Schizophrenia Research·S Hossein FatemiRobert W Sidwell
Apr 8, 2015·Chromosoma·M ArroyoJ A Marchal
Mar 10, 2009·American Journal of Medical Genetics. Part a·Reycel Maghirang-RodriguezJulianne S Collins
Sep 24, 2010·Annals of the New York Academy of Sciences·Bernard J Crespi
Nov 11, 2010·Annals of the New York Academy of Sciences·Tracy J Dixon-Salazar, Joseph G Gleeson

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.